ArticleCell systems2020
Growth-Optimized Aminoacyl-tRNA Synthetase Levels Prevent Maximal tRNA Charging.
Article in Cell systems, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Environmental Stress Induces Altered Composition of Streptococcus mutans Membrane Vesicles: pH-Driven Changes in Membrane Vesicle Production and Composition.Molecular oral microbiology · 2026Article
- Availability of Charged tRNAs Drives Maximal Protein Synthesis at Intermediate Levels of Codon Usage Bias.Bulletin of mathematical biology · 2026Article
- DEAD-box ATPase-marked condensates coordinate compartmentalized translation and antibiotic persistence.Science advances · 2026Article
- Lipidomics combined with amino acids and their derivatives: Targeted metabolomics analysis for the quality deterioration of coconut water.Current research in food science · 2026Article
- Analysis of (p)ppGpp metabolism and signaling using a dynamic luminescent reporter.PLoS genetics · 2025Article
- A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis.Nucleic acids research · 2025Article
- How DoesACS synthetic biology · 2024Article
- Deciphering the Intracellular Action of the Antimicrobial Peptide A11 via an In-Depth Analysis of Its Effect on the Global Proteome ofACS infectious diseases · 2024Article
- Pairing metagenomics and metaproteomics to characterize ecological niches and metabolic essentiality of gut microbiomes.ISME communications · 2024Article
- Genetically stable kill-switch using "demon and angel" expression construct of essential genes.Frontiers in bioengineering and biotechnology · 2024Article
- Atlas of mRNA translation and decay for bacteria.Nature microbiology · 2023Article
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- Ubiquitous mRNA decay fragments in E. coli redefine the functional transcriptome.Nucleic acids research · 2022Article
- Crosstalk between guanosine nucleotides regulates cellular heterogeneity in protein synthesis during nutrient limitation.PLoS genetics · 2022Article
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- Proteomic traits vary across taxa in a coastal Antarctic phytoplankton bloom.The ISME journal · 2022Article
- Quantitative Control for Stoichiometric Protein Synthesis.Annual review of microbiology · 2021Article
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- Fundamental limits on the rate of bacterial growth and their influence on proteomic composition.Cell systems · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Aminoacyl-tRNA synthetases (aaRSs) serve a dual role in charging tRNAs. Their enzymatic activities both provide protein synthesis flux and reduce uncharged tRNA levels. Although uncharged tRNAs can negatively impact bacterial growth, substantial concentrations of tRNAs remain deacylated even under nutrient-rich conditions. Here, we show that tRNA charging in Bacillus subtilis is not maximized due to optimization of aaRS production during rapid growth, which prioritizes demands in protein synthesis over charging levels. The presence of uncharged tRNAs is alleviated by precisely tuned translation kinetics and the stringent response, both insensitive to aaRS overproduction but sharply responsive to underproduction, allowing for just enough aaRS production atop a "fitness cliff." Notably, we find that the stringent response mitigates fitness defects at all aaRS underproduction levels even without external starvation. Thus, adherence to minimal, flux-satisfying protein production drives limited tRNA charging and provides a basis for the sensitivity and setpoints of an integrated growth-control network.
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