ArticleAmerican journal of physiology. Lung cellular and molecular physiology2020
Normal mesothelial cell lines newly derived from human pleural biopsy explants.
Article in American journal of physiology. Lung cellular and molecular physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Cytotoxic Microspinosamides from the Marine SpongeMarine drugs · 2026Article
- Pure isolation, culture, and postinjury lineage tracing of mouse visceral pleural mesothelial cells.American journal of respiratory cell and molecular biology · 2026Article
- Pulmonary organoid models demonstrate compositionally driven epithelial plasticity and immune polarization.iScience · 2026Article
- Identification of Cembrane Diterpenoids fromJournal of natural products · 2025Article
- microRNA-497-5p-based screening identifies a novel synthetic lethal-type interaction via PKMYT1 and WEE1 in pleural mesothelioma.Molecular therapy. Nucleic acids · 2025Article
- Sesquiterpene Coumarin Ethers and Phenylpropanoids from the Roots ofMolecules (Basel, Switzerland) · 2025Article
- FGF2 promotes the expansion of parietal mesothelial progenitor pools and inhibits BMP4-mediated smooth muscle cell differentiation.Frontiers in cell and developmental biology · 2024Article
- Single-cell transcriptomic analysis of human pleura reveals stromal heterogeneity and informsThe European respiratory journal · 2024Article
- Targeting FAcilitates Chromatin Transcription complex inhibits pleural mesothelioma and enhances immunotherapy.Journal of experimental & clinical cancer research : CR · 2023Article
- Article
- Cancer-associated mesothelial cell-derived ANGPTL4 and STC1 promote the early steps of ovarian cancer metastasis.JCI insight · 2023Article
- Headspace Volatile Organic Compound Profiling of Pleural Mesothelioma and Lung Cancer Cell Lines as Translational Bridge for Breath Research.Frontiers in oncology · 2022Article
- Article
- MicroRNA-206 suppresses mesothelioma progression via the Ras signaling axis.Molecular therapy. Nucleic acids · 2021Article
- Folliculin haploinsufficiency causes cellular dysfunction of pleural mesothelial cells.Scientific reports · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mesothelial cells are arranged as a monolayer on covering membranes that invest surfaces of body cavities like the pleura and peritoneum. Primary human mesothelial cell (HMC) cultures are needed for studying mesothelial cell homeostasis and developing disease models, such as wound healing or cancers. Remarkably, there is a paucity of useable HMC lines that are currently available that faithfully recapitulate normal in vivo phenotypic characteristics. Here, we present a strategy to recover HMC from human pleural tissue and to immortalize them for extended in vitro culturing. Human pleural membrane was harvested by minimally invasive surgical techniques. HMC were isolated using a two-step process combining explant cellular outgrowth from biopsy tissue and flow cytometry based on cell surface expression of cadherin-1 and CD71. Cell cultures were generated after lentiviral transfection with human telomerase. The new HMC cultures retain the same phenotypic traits and physiologic features as their in vivo counterparts, yet they can be adapted for short-term or long-term culture in large-scale in vitro experimentation. In particular, we generated a new HMC line harboring a germline mutation in breast cancer type-1-associated protein-1 (
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