Evidence map›Paper›PMID 32725914›Full record

ArticleChemistry (Weinheim an der Bergstrasse, Germany)2020

An Evolutionary Strategy for Identification of Higher Order, Green Fluorescent Host-Guest Pairs Compatible with Living Systems.

Garrett R Casey, Xinqi Zhou, Lauren Lesiak, Bi Xu, Yuan Fang, Donald F Becker, Cliff I Stains

Abstract read
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In one paragraph

Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Garrett R CaseyDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Xinqi ZhouDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Lauren LesiakDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Bi XuDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Yuan FangDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Donald F BeckerDepartment of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Cliff I StainsDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.ORCID http://orcid.org/0000-0002-3165-4539

Funding

Chemical Approaches for Interrogating Fundamental Biomedical ProcessesR35GM119751 · NIGMS · UNIVERSITY OF VIRGINIA · PI STAINS, CLIFF I · 2016 to 2020
$1.8M
NIGMS NIH HHS R35 GM119751NIGMS NIH HHS R35GM119751
6 · The paper itself

Abstract

Engineered miniprotein host-small-molecule guest pairs could be utilized to design new processes within cells as well as investigate fundamental aspects of cell signaling mechanisms. However, the development of host-guest pairs capable of functioning in living systems has proven challenging. Moreover, few examples of host-guest pairs with stoichiometries other than 2:1 exist, significantly hindering the ability to study the influence of oligomerization state on signaling fidelity. Herein, we present an approach to identify host-guest systems for relatively small green fluorescent guests by incorporation into cyclic peptides. The optimal host-guest pair produced a 10-fold increase in green fluorescence signal upon binding. Biophysical characterization clearly demonstrated higher order supramolecular assembly, which could be visualized on the surface of living yeast cells using a turn-on fluorescence readout. This work further defines evolutionary design principles to afford host-guest pairs with stoichiometries other than 2:1 and enables the identification of spectrally orthogonal host-guest pairs.

Indexed as

ColorFluorescenceSaccharomyces cerevisiaeBiocompatible MaterialsPeptides, CyclicProtein BindingBiocompatible MaterialsPeptides, Cyclicdirected evolutionfluorescent probeshost-guest systemsprotein engineeringself-assembly

Identifiers

PMID32725914
PMCPMC7744332

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.