ArticlePlanta2020
Involvement of CsERF2 in leaf variegation of Cymbidium sinense 'Dharma'.
Article in Planta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Loss of the nuclear factor Y subunit B3 gene causes chlorosis and chloroplast development defects in cucumber.Horticulture research · 2026Article
- Genome-wide analysis of AP2/ERF transcription Factors inFrontiers in plant science · 2025Article
- Integrating Physiology, Cytology, and Transcriptome to Reveal the Leaf Variegation Mechanism inBiomolecules · 2024Article
- Molecular Mechanisms of Chlorophyll Deficiency inPlants (Basel, Switzerland) · 2024Article
- Fine Mapping of BoVl Conferring the Variegated Leaf in Ornamental Kale (Brassica oleracea var. acephala)International journal of molecular sciences · 2022Article
- Insight into the molecular mechanisms of leaf coloration inFrontiers in genetics · 2022Article
- The genome of Cymbidium sinense revealed the evolution of orchid traits.Plant biotechnology journal · 2021Article
- Cytological and Transcriptomic Analysis Provide Insights into the Formation of Variegated Leaves inPlants (Basel, Switzerland) · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
MAIN
conclusionCsERF2, an ethylene response factor, plays a role in leaf variegation. Leaf variegation is a main ornamental characteristic in Cymbidium sinense (C. sinense). However, the mechanisms of leaf color variegation remain largely unclear. In the present study, we analyzed the cytological and physiological features, as well as molecular analyses of leaves from wild-type (WT) and leaf variegation mutants of Cymbidium sinense 'Dharma'. Chloroplasts with typical and functional structures were discovered in WT and green sectors of the mutants leaves (MG), but not in yellow sectors of the mutant leaves (MY). The activities of key enzymes involved in chlorophyll (Chl) degradation and their substrate contents were significantly increased in MY. Genes related to Chl degradation also showed a significant up-regulation in MY. Transcriptomic analysis showed that the expression of all identified ethylene response factors (ERFs) was significantly up-regulated, and the 1-aminocyclopropane-1-carboxylic acid (ACC) content in MY was significantly higher compared with MG. QRT-PCR analysis validated that the expression levels of genes related to Chl degradation could be positively affected by ethylene (ETH) treatment. Stable overexpression of CsERF2 in Nicotiana tabacum (N. tabacum) led to a decrease in Chl content and abnormal chloroplast. Transcriptomic analysis and qRT-PCR results showed that the KEGG pathway related to chloroplast development and function showed significant change in transgenic N. tabacum. Therefore, the leaf color formation of C. sinense was greatly affected by chloroplast development and Chl metabolism. CsERF2 played an important role in leaf variegation of C. sinense.
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