ArticleBMC materials2020
Optimization of skeletal protein preparation for LC-MS/MS sequencing yields additional coral skeletal proteins in
Article in BMC materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Evolutionary rewiring of the endomesoderm gene regulatory networks specify skeleton secreting cells in stony corals.bioRxiv : the preprint server for biology · 2026Article
- Coral larval proteomics at onset of metamorphosis highlights innate immunity maturation in parallel to neuro-sensing and skeletal development.Frontiers in physiology · 2026Article
- Spatially resolved gene expression analysis illuminates location-specific functions in the reef-building coral Pocillopora acuta.PloS one · 2026Article
- Article
- In-depth single-cell transcriptomic exploration of the regenerative dynamics in stony coral.Communications biology · 2025Article
- The effects of seawater temperature-induced coral bleaching on the aragonite structure and material properties of massiveCoral reefs (Online) · 2025Article
- Extreme Environmental Variability Induces Frontloading of Coral Biomineralisation Genes to Maintain Calcification Under pCOMolecular ecology · 2025Article
- Genomic signatures suggesting adaptation to ocean acidification in a coral holobiont from volcanic COCommunications biology · 2023Article
- First paleoproteome study of fossil fish otoliths and the pristine preservation of the biomineral crystal host.Scientific reports · 2023Article
- Deep resilience: An evolutionary perspective on calcification in an age of ocean acidification.Frontiers in physiology · 2023Article
- Utilizing an artificial intelligence system to build the digital structural proteome of reef-building corals.GigaScience · 2022Article
- Different skeletal protein toolkits achieve similar structure and performance in the tropical coral Stylophora pistillata and the temperate Oculina patagonica.Scientific reports · 2022Article
- Mechanisms and potential immune tradeoffs of accelerated coral growth induced by microfragmentation.PeerJ · 2022Article
- The Skeleton and Biomineralization Mechanism as Part of the Innate Immune System of Stony Corals.Frontiers in immunology · 2022Review
- Gene expression associated with disease resistance and long-term growth in a reef-building coral.Royal Society open science · 2021Article
- The spatial network of skeletal proteins in a stony coral.Journal of the Royal Society, Interface · 2021Article
- Article
- Evolution of Protein-Mediated Biomineralization in Scleractinian Corals.Frontiers in genetics · 2021Article
- First sequencing of ancient coral skeletal proteins.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Stony corals generate their calcium carbonate exoskeleton in a highly controlled biomineralization process mediated by a variety of macromolecules including proteins. Fully identifying and classifying these proteins is crucial to understanding their role in exoskeleton formation, yet no optimal method to purify and characterize the full suite of extracted coral skeletal proteins has been established and hence their complete composition remains obscure. Here, we tested four skeletal protein purification protocols using acetone precipitation and ultrafiltration dialysis filters to present a comprehensive scleractinian coral skeletal proteome. We identified a total of 60 proteins in the coral skeleton, 44 of which were not present in previously published stony coral skeletal proteomes. Extracted protein purification protocols carried out in this study revealed that no one method captures all proteins and each protocol revealed a unique set of method-exclusive proteins. To better understand the general mechanism of skeletal protein transportation, we further examined the proteins' gene ontology, transmembrane domains, and signal peptides. We found that transmembrane domain proteins and signal peptide secretion pathways, by themselves, could not explain the transportation of proteins to the skeleton. We therefore propose that some proteins are transported to the skeleton via non-traditional secretion pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.