ArticleEcology and evolution2020
In silico and empirical evaluation of twelve metabarcoding primer sets for insectivorous diet analyses.
Article in Ecology and evolution, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 62 citations in OpenAlex.
- Mitogenome-Informed Metabarcoding for Non-Invasive Identification of Sympatric Flamingos in Northern Chile.Biology · 2026Article
- Understanding biological control function and trophic interaction dynamics of an artificially released predatory bug by DNA metabarcoding.Insect science · 2026Article
- Urbanization Shapes Dietary Composition and Snout Morphology of Salamanders.Molecular ecology · 2026Article
- Article
- Dietary DNA Metabarcoding From Animal Fecal Samples.Current protocols · 2026Article
- Ecological Characteristics of Large-Bodied Sharks in the East Sea of Korea.Animals : an open access journal from MDPI · 2025Article
- Metabarcoding Reveals the Dietary Patterns of Bats Within a Unique European Habitat, the Pasture-Dominated Landscapes of Ireland.Ecology and evolution · 2025Article
- Evaluation of Oxford Nanopore Technologies MinION Sequencer as a Novel Short Amplicon Metabarcoding Tool Using Arthropod Mock Sample and Irish Bat Diet Characterisation.Ecology and evolution · 2025Article
- The impact of wildfires on the diet of Podarcis lusitanicus revealed by DNA metabarcoding.PloS one · 2025Article
- All-you-can-eat buffet: A spider-specialized bat species (Myotis emarginatus) turns into a pest fly eater around cattle.PloS one · 2024Article
- The predator problem and PCR primers in molecular dietary analysis: Swamped or silenced; depth or breadth?Molecular ecology resources · 2023Review
- DNA metabarcoding suggests adaptive seasonal variation of individual trophic traits in a critically endangered fish.Molecular ecology · 2022Article
- Addressing nontarget amplification in DNA metabarcoding studies of arthropod-feeding rodents.Mammal research · 2022Article
- Food for everyone: Differential feeding habits of cryptic bat species inferred from DNA metabarcoding.Molecular ecology · 2021Article
- In silico and empirical evaluation of twelve metabarcoding primer sets for insectivorous diet analyses.Ecology and evolution · 2020Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the most recent decade, environmental DNA metabarcoding approaches have been both developed and improved to minimize the biological and technical biases in these protocols. However, challenges remain, notably those relating to primer design. In the current study, we comprehensively assessed the performance of ten COI and two 16S primer pairs for eDNA metabarcoding, including novel and previously published primers. We used a combined approach of in silico, in vivo-mock community (33 arthropod taxa from 16 orders), and guano-based analyses to identify primer sets that would maximize arthropod detection and taxonomic identification, successfully identify the predator (bat) species, and minimize the time and financial costs of the experiment. We focused on two insectivorous bat species that live together in mixed colonies: the greater horseshoe bat (
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