ArticleScientific reports2020
Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- Application of fluorescence micro-optical sectioning tomography in the cerebrovasculature and applicable vascular labeling methods.Brain structure & function · 2023Review
- A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.Cell reports methods · 2023Article
- Long-term and non-invasiveWorld journal of hepatology · 2022Article
- Optimal combinations of fluorescent vessel labeling and tissue clearing methods for three-dimensional visualization of vasculature.Neurophotonics · 2022Article
- Lamina cribrosa vessel and collagen beam networks are distinct.Experimental eye research · 2022Article
- Complex-Amplitude-Modulation Vectorial Excitation Beam for High-Resolution Observation of Deep Regions in Two-Photon Microscopy.Frontiers in neuroscience · 2022Article
- High-Resolution Digital Panorama of Multiple Structures in Whole Brain of Alzheimer's Disease Mice.Frontiers in neuroscience · 2022Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simultaneous visualisation of vasculature and surrounding tissue structures is essential for a better understanding of vascular pathologies. In this work, we describe a histochemical strategy for three-dimensional, multicolour imaging of vasculature and associated structures, using a carbocyanine dye-based technique, vessel painting. We developed a series of applications to allow the combination of vessel painting with other histochemical methods, including immunostaining and tissue clearing for confocal and two-photon microscopies. We also introduced a two-photon microscopy setup that incorporates an aberration correction system to correct aberrations caused by the mismatch of refractive indices between samples and immersion mediums, for higher-quality images of intact tissue structures. Finally, we demonstrate the practical utility of our approach by visualising fine pathological alterations to the renal glomeruli of IgA nephropathy model mice in unprecedented detail. The technical advancements should enhance the versatility of vessel painting, offering rapid and cost-effective methods for vascular pathologies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.