Evidence map›Paper›PMID 32723478›Full record

ArticleeLife2020

Accurate and versatile 3D segmentation of plant tissues at cellular resolution.

Adrian Wolny, Lorenzo Cerrone, Athul Vijayan, Rachele Tofanelli, Amaya Vilches Barro, Marion Louveaux, Christian Wenzl, Sören Strauss, David Wilson-Sánchez, Rena Lymbouridou and 11 more

Abstract read
In one paragraph

Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.

0numbers the graph read from it
0cells of the map it votes in
136citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

136 citing papers in PubMed.

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  17. ICECREAM: high-fidelity equivariant cryo-electron tomography.Acta crystallographica. Section D, Structural biology · 2026
    Article
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  19. Article
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76 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Adrian Wolny *Heidelberg Collaboratory for Image Processing, Heidelberg University, Heidelberg, Germany.ORCID 0000-0003-2794-4266
Lorenzo Cerrone *Heidelberg Collaboratory for Image Processing, Heidelberg University, Heidelberg, Germany.
Athul VijayanSchool of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany.
Rachele TofanelliSchool of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany.ORCID 0000-0002-5196-1122
Amaya Vilches BarroCentre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
Marion LouveauxCentre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
Christian WenzlCentre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
Sören StraussDepartment of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
David Wilson-SánchezDepartment of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Rena LymbouridouDepartment of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Susanne S SteiglederCentre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
Constantin PapeHeidelberg Collaboratory for Image Processing, Heidelberg University, Heidelberg, Germany.
Alberto BailoniHeidelberg Collaboratory for Image Processing, Heidelberg University, Heidelberg, Germany.
Salva Duran-NebredaSchool of Life Sciences, University of Warwick, Coventry, United Kingdom.
George W BasselSchool of Life Sciences, University of Warwick, Coventry, United Kingdom.
Jan U LohmannCentre for Organismal Studies, Heidelberg University, Heidelberg, Germany.ORCID 0000-0003-3667-187X
Miltos TsiantisDepartment of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Fred A HamprechtHeidelberg Collaboratory for Image Processing, Heidelberg University, Heidelberg, Germany.
Kay SchneitzSchool of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany.ORCID 0000-0001-6688-0539
Alexis MaizelCentre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
Anna KreshukEMBL, Heidelberg, Germany.ORCID 0000-0003-1334-6388

Funding

Deutsche Forschungsgemeinschaft FOR2581Leverhulme Trust RPG-2016-049
6 · The paper itself

Abstract

Quantitative analysis of plant and animal morphogenesis requires accurate segmentation of individual cells in volumetric images of growing organs. In the last years, deep learning has provided robust automated algorithms that approach human performance, with applications to bio-image analysis now starting to emerge. Here, we present PlantSeg, a pipeline for volumetric segmentation of plant tissues into cells. PlantSeg employs a convolutional neural network to predict cell boundaries and graph partitioning to segment cells based on the neural network predictions. PlantSeg was trained on fixed and live plant organs imaged with confocal and light sheet microscopes. PlantSeg delivers accurate results and generalizes well across different tissues, scales, acquisition settings even on non plant samples. We present results of PlantSeg applications in diverse developmental contexts. PlantSeg is free and open-source, with both a command line and a user-friendly graphical interface.

Indexed as

Plant CellsSoftwareArabidopsisImaging, Three-DimensionalNeural Networks, ComputerA. thalianacell segmentationdeep learningimage analysisinstance segmentationplant biology

Identifiers

PMID32723478
PMCPMC7447435

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.