Evidence map›Paper›PMID 32719956›Full record

ArticleArchives of virology2020

Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses.

Tomoichiro Oka, Seiji P Yamamoto, Nobuhiro Iritani, Shigenori Sato, Chika Tatsumi, Tetsuo Mita, Shunsuke Yahiro, Shinichiro Shibata, Fang-Tzy Wu, Hirotaka Takagi

Open access · bronzeAbstract read
In one paragraph

Article in Archives of virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.2field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Tomoichiro OkaDepartment of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo, 208-0011, Japan. oka-t@nih.go.jp.ORCID http://orcid.org/0000-0001-5224-2499
Seiji P YamamotoDivision of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
Nobuhiro IritaniDivision of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
Shigenori SatoDivision of Virology and Medical Zoology, Chiba Prefectural Institute of Public Health, Chiba, Japan.
Chika TatsumiDivision of Virology, Shimane Prefectural Institute of Public Health and Environmental Science, Shimane, Japan.
Tetsuo MitaDivision of Virology, Shimane Prefectural Institute of Public Health and Environmental Science, Shimane, Japan.
Shunsuke YahiroDepartment of Microbiology, Kumamoto Prefectural Institute of Public Health and Environmental Science, Kumamoto, Japan.
Shinichiro ShibataMicrobiology Department, Nagoya City Public Health Research Institute, Aichi, Japan.
Fang-Tzy WuCenter for Research, Diagnostics and Vaccine Development, Taiwan Centers for Disease Control, Taipei, Taiwan.
Hirotaka TakagiManagement Department of Biosafety and Laboratory Animal, National Institute of Infectious Diseases, Tokyo, Japan.
National Institute of Infectious Diseases · JPOsaka Prefectural Institute of Public Health · JPShimane Prefectural Institute of Public Health and Environmental Sciences · JPChiba Prefectural Institute of Public Health · JPDevelopment Center for Biotechnology · TWKumamoto Health Science University · JP

Funding

Japan Agency for Medical Research and Development JP19fk0108049Japan Agency for Medical Research and Development JP20fk0108139
6 · The paper itself

Abstract

Sapoviruses are increasingly being recognized as pathogens associated with gastroenteritis in humans. Human sapoviruses are currently assigned to 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the sequence of the region encoding the major structural protein. In this study, we evaluated 11 polymerase chain reaction (PCR) assays using published and newly designed/modified primers and showed that four PCR assays with different primer combinations amplified all of the tested human sapovirus genotypes using either synthetic DNA or cDNA prepared from human sapovirus-positive fecal specimens. These assays can be used as improved broadly reactive screening tests or as tools for molecular characterization of human sapoviruses.

Indexed as

Base SequenceCaliciviridae InfectionsDNA PrimersFecesGastroenteritisGene ExpressionGenotypeHumansMolecular TypingPhylogenyReverse Transcriptase Polymerase Chain ReactionSapovirusSequence AlignmentViral Structural ProteinsDNA PrimersViral Structural Proteins

Identifiers

PMID32719956
PMCPMC7383071
OpenAlexW3045899919

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.