ArticleThe Journal of antimicrobial chemotherapy2020
Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages.
Article in The Journal of antimicrobial chemotherapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2026Review
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Role of Lysogenic Phages in the Dissemination of Antibiotic Resistance Genes Applied in the Food Chain.Foods (Basel, Switzerland) · 2025Review
- Hijackers, hitchhikers, or co-drivers? The mysteries of mobilizable genetic elements.PLoS biology · 2024Article
- Structure and dispersion of the conjugative mobilome in surface ocean bacterioplankton.ISME communications · 2024Article
- Metagenomic investigation reveals bacteriophage-mediated horizontal transfer of antibiotic resistance genes in microbial communities of an organic agricultural ecosystem.Microbiology spectrum · 2023Article
- The Role of Temperate Phages in Bacterial Pathogenicity.Microorganisms · 2023Review
- Dominance of phage particles carrying antibiotic resistance genes in the viromes of retail food sources.The ISME journal · 2023Article
- Selfish, promiscuous and sometimes useful: how mobile genetic elements drive horizontal gene transfer in microbial populations.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Antimicrobial resistance and One Health.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2022Review
- Genome sequencing and comparative analysis of Wolbachia strain wAlbA reveals Wolbachia-associated plasmids are common.PLoS genetics · 2022Article
- Inhibitory activities of propolis, nisin, melittin and essential oil compounds onThe journal of venomous animals and toxins including tropical diseases · 2022Article
- Plasmids do not consistently stabilize cooperation across bacteria but may promote broad pathogen host-range.Nature ecology & evolution · 2021Article
- Evolution of ColE1-like plasmids across γ-Proteobacteria: From bacteriocin production to antimicrobial resistance.PLoS genetics · 2021Article
- Bacteriophages of Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2021Review
- The Age of Phage: Friend or Foe in the New Dawn of Therapeutic and Biocontrol Applications?Pharmaceuticals (Basel, Switzerland) · 2021Review
- Risk of Bacteriophage Therapeutics to Transfer Genetic Material and Contain Contaminants Beyond Endotoxins with Clinically Relevant Mitigation Strategies.Infection and drug resistance · 2021Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles.
methodsSeveral databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a marker in eight different plasmids, from different incompatibility groups, and with differing sizes and plasmid copy numbers. All plasmids were transformed into Escherichia coli bearing one of four different lysogenic phages. Upon induction, encapsidation of armA in phage particles was evaluated using qRT-PCR and Southern blotting.
resultsMulticopy plasmids carry a vast set of emerging clinically important antimicrobial resistance genes. However, 60% of these plasmids do not bear mobility (MOB) genes. When carried on these multicopy plasmids, mobilization of a marker gene armA into phage capsids was up to 10000 times more frequent than when it was encoded by a large plasmid with a low copy number.
conclusionsMulticopy plasmids and phages, two major mobile genetic elements (MGE) in bacteria, represent a novel high-efficiency transmission route of antimicrobial resistance genes that deserves further investigation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.