ArticleNature methods2020
A general method to optimize and functionalize red-shifted rhodamine dyes.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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Who cites it
123 citing papers in PubMed.
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- A series of spontaneously blinking dyes for super-resolution microscopy.Nature methods · 2026Article
- Painting dopamine in far-red: a chemigenetic breakthrough.Science China. Life sciences · 2026Article
- Single-Molecule Fluorescence Defines a New Dye Chemistry.Chemical & biomedical imaging · 2026Review
- Optimized Xanthenium Photocages with Fused Ring Systems for Photoactivated Chemotherapy and G Protein-Coupled Receptor Photopharmacology.Journal of the American Chemical Society · 2026Article
- Article
- Monitoring in real time and far-red imaging of HNature chemical biology · 2026Article
- Molecular Design with Artificial Intelligence: Progress and Perspectives for Small Molecules.Chemical reviews · 2026Review
- Lewis Acid-Triggered Spatiotemporally Controllable Ring Opening in a Classic Rhodamine Featuring Φ = 95% Emission.Small science · 2026Article
63 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors.
Funding
Abstract
Expanding the palette of fluorescent dyes is vital to push the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore owing to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a framework for rationalizing rhodamine behavior in biological environments and a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups. This strategy yields red-shifted 'Janelia Fluor' (JF) dyes useful for biological imaging experiments in cells and in vivo.
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