Evidence map›Paper›PMID 32719404›Full record

ArticleScientific reports2020

Antibody drug separation using thermoresponsive anionic polymer brush modified beads with optimised electrostatic and hydrophobic interactions.

Kenichi Nagase, Saki Ishii, Koji Ikeda, Sota Yamada, Daiju Ichikawa, Aya Mizutani Akimoto, Yutaka Hattori, Hideko Kanazawa

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 48 citations in OpenAlex.

  1. Temperature-modulated separation of therapeutic cells, viral vectors, and exosomes using functional polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
  2. Review
  3. Article
  4. Current advances in separation chemistry for antibody purification and analysis.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
  5. Molecular design of dynamically thermoresponsive biomaterials.Science and technology of advanced materials · 2025
    Review
  6. Article
  7. Article
  8. Article
  9. Review
  10. Bioanalytical technologies using temperature-responsive polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024
    Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Kenichi NagaseFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Tokyo, Minato, 105-8512, Japan. nagase-kn@pha.keio.ac.jp.
Saki IshiiFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Tokyo, Minato, 105-8512, Japan.
Koji IkedaFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Tokyo, Minato, 105-8512, Japan.
Sota YamadaFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Tokyo, Minato, 105-8512, Japan.
Daiju IchikawaFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Tokyo, Minato, 105-8512, Japan.
Aya Mizutani AkimotoDepartment of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan.
Yutaka HattoriFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Tokyo, Minato, 105-8512, Japan.
Hideko KanazawaFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Tokyo, Minato, 105-8512, Japan.
Keio University · JPThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibody drugs play an important role in biopharmaceuticals, because of the specificity for target biomolecules and reduction of side effects. Thus, separation and analysis techniques for these antibody drugs have increased in importance. In the present study, we develop functional chromatography matrices for antibody drug separation and analysis. Three types of polymers, poly(N-isopropylacrylamide (NIPAAm)-co-2-acrylamido-2-methylpropanesulfonic acid (AMPS)-co-N-phenyl acrylamide (PhAAm)), P(NIPAAm-co-AMPS-co-n-butyl methacrylate (BMA)), and P(NIPAAm-co-AMPS-co-tert-butylacrylamide (tBAAm)), were modified on silica beads through atom transfer radical polymerisation. Rituximab elution profiles were observed using the prepared beads-packed column. Rituximab adsorption at high temperature and elution at low temperature from the column were observed, as a result of the temperature-modulated electrostatic and hydrophobic interactions. Using the column, rituximab purification from contaminants was performed simply by changing the temperature. Additionally, three types of antibody drugs were separated using the column through temperature-modulated hydrophobic and electrostatic interactions. These results demonstrate that the temperature-responsive column can be applied for the separation and analysis of biopharmaceuticals through a simple control of the column temperature.

Indexed as

MicrospheresAcrylamidesAdsorptionAnionsAntibodies, MonoclonalBevacizumabCetuximabChromatography, Ion ExchangeDihydroxyphenylalanineEpinephrineHydrophobic and Hydrophilic InteractionsMicroscopy, Electron, ScanningPharmaceutical PreparationsPolymersRituximabSilicon DioxideAcrylamidesAnionsAntibodies, MonoclonalBevacizumabCetuximabDihydroxyphenylalanineEpinephrinePharmaceutical PreparationsPolymersRituximabSilicon Dioxide

Identifiers

PMID32719404
PMCPMC7385495
OpenAlexW3045211739

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.