Evidence map›Paper›PMID 32719001›Full record

ArticleScience translational medicine2020

A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples.

Viet Loan Dao Thi, Konrad Herbst, Kathleen Boerner, Matthias Meurer, Lukas Pm Kremer, Daniel Kirrmaier, Andrew Freistaedter, Dimitrios Papagiannidis, Carla Galmozzi, Megan L Stanifer and 9 more

Open access · hybridAbstract readValidation Study
In one paragraph

Article in Science translational medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 369 papers.

0numbers the graph read from it
0cells of the map it votes in
369citing papers in PubMed
48.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

369 citing papers in PubMed, 696 citations in OpenAlex.

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  2. Cost and cost-effectiveness of four different SARS-CoV-2 active surveillance strategies: evidence from a randomised control trial in Germany.The European journal of health economics : HEPAC : health economics in prevention and care · 2023
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  18. Standardization and performance of SARS-CoV-2 RT-LAMP detection: a reliable, inexpensive, and alternative diagnostic assay.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025
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309 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 1 country.

Viet Loan Dao ThiSchaller Research Groups, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany. vietloan.daothi@med.uni-heidelberg.de m.knop@zmbh.uni-heidelberg.de s.anders@zmbh.uni-heidelberg.de.ORCID 0000-0003-2293-3592
Konrad HerbstCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.ORCID 0000-0003-1296-1522
Kathleen BoernerDepartment of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.
Matthias MeurerCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.ORCID 0000-0003-4651-128X
Lukas Pm KremerCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.ORCID 0000-0003-3170-6295
Daniel KirrmaierCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.
Andrew FreistaedterSchaller Research Groups, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.ORCID 0000-0002-9374-1056
Dimitrios PapagiannidisCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.ORCID 0000-0001-5650-6949
Carla GalmozziCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.ORCID 0000-0002-1415-6757
Megan L StaniferDepartment of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.ORCID 0000-0002-5606-1297
Steeve BoulantDepartment of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.ORCID 0000-0001-8614-4993
Steffen KleinSchaller Research Groups, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.ORCID 0000-0002-8046-0474
Petr ChlandaSchaller Research Groups, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.ORCID 0000-0002-7782-2139
Dina KhalidDepartment of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.ORCID 0000-0002-8845-2428
Isabel Barreto MirandaDepartment of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.
Paul SchnitzlerDepartment of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.
Hans-Georg KräusslichDepartment of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.ORCID 0000-0002-8756-329X
Michael KnopCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany. vietloan.daothi@med.uni-heidelberg.de m.knop@zmbh.uni-heidelberg.de s.anders@zmbh.uni-heidelberg.de.ORCID 0000-0003-2566-923X
Simon AndersCenter for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany. vietloan.daothi@med.uni-heidelberg.de m.knop@zmbh.uni-heidelberg.de s.anders@zmbh.uni-heidelberg.de.ORCID 0000-0003-4868-1805
Heidelberg University · DEGerman Cancer Research Center · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) coronavirus is a major public health challenge. Rapid tests for detecting existing SARS-CoV-2 infections and assessing virus spread are critical. Approaches to detect viral RNA based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) have potential as simple, scalable, and broadly applicable testing methods. Compared to RT quantitative polymerase chain reaction (RT-qPCR)-based methods, RT-LAMP assays require incubation at a constant temperature, thus eliminating the need for sophisticated instrumentation. Here, we tested a two-color RT-LAMP assay protocol for detecting SARS-CoV-2 viral RNA using a primer set specific for the

Indexed as

BetacoronavirusColorimetryCoronavirus InfectionsCoronavirus Nucleocapsid ProteinsCOVID-19HumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesNucleocapsid ProteinsPandemicsPhosphoproteinsPneumonia, ViralRNA-SeqRNA, ViralSARS-CoV-2Sensitivity and SpecificityCoronavirus Nucleocapsid Proteinsnucleocapsid phosphoprotein, SARS-CoV-2Nucleocapsid ProteinsPhosphoproteinsRNA, Viral

Identifiers

PMID32719001
PMCPMC7574920
OpenAlexW3045178645

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.