ArticleiScience2020
Two-Photon Voltage Imaging of Spontaneous Activity from Multiple Neurons Reveals Network Activity in Brain Tissue.
Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo.Nature methods · 2026Article
- The axon initial segment-associated microglia regulate neuronal activity and visual perception.Cell research · 2026Article
- Article
- Neuronal 'Ensemble' Recording and the Search for the Cell Assembly: A Personal History.Hippocampus · 2025Review
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- Scanless two-photon voltage imaging.Nature communications · 2024Article
- Large-scale deep tissue voltage imaging with targeted-illumination confocal microscopy.Nature methods · 2024Article
- Brain orchestra under spontaneous conditions: Identifying communication modules from the functional architecture of area V1.bioRxiv : the preprint server for biology · 2024Article
- Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy.Nature communications · 2023Article
- Diversity of dynamic voltage patterns in neuronal dendrites revealed by nanopipette electrophysiology.Nanoscale · 2023Article
- Large-scale deep tissue voltage imaging with targeted illumination confocal microscopy.bioRxiv : the preprint server for biology · 2023Article
- Current Practice in Using Voltage Imaging to Record Fast Neuronal Activity: Successful Examples from Invertebrate to Mammalian Studies.Biosensors · 2023Review
- Article
- Scanless two-photon voltage imaging.Research square · 2023Article
- Advantages, Pitfalls, and Developments of All Optical Interrogation Strategies of MicrocircuitsFrontiers in neuroscience · 2022Review
- Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo.Nature communications · 2021Article
- A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recording the electrical activity of multiple neurons simultaneously would greatly facilitate studies on the function of neuronal circuits. The combination of the fast scanning by random-access multiphoton microscopy (RAMP) and the latest two-photon-compatible high-performance fluorescent genetically encoded voltage indicators (GEVIs) has enabled action potential detection in deep layers in in vivo brain. However, neuron connectivity analysis on optically recorded action potentials from multiple neurons in brain tissue has yet to be achieved. With high expression of a two-photon-compatible GEVI, ASAP3, via in utero electroporation and RAMP, we achieved voltage recording of spontaneous activities from multiple neurons in brain slice. We provide evidence for the developmental changes in intralaminar horizontal connections in somatosensory cortex layer 2/3 with a greater sensitivity than calcium imaging. This method thus enables investigation of neuronal network connectivity at the cellular resolution in brain tissue.
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