ArticlePloS one2020
Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 46 citations in OpenAlex.
- Functional Dipeptide Production by Immobilized Enzyme on Yeast Cell Surface.Journal of microbiology and biotechnology · 2026Article
- Engineering the acid-tolerant yeastFrontiers in microbiology · 2026Article
- Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass.Journal of microbiology (Seoul, Korea) · 2025Review
- Advances in the biological production of sugar alcohols from biomass-derived xylose.World journal of microbiology & biotechnology · 2025Review
- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Article
- Construction of an economical xylose-utilizing Saccharomyces cerevisiae and its ethanol fermentation.FEMS yeast research · 2024Article
- Metabolic engineering of Saccharomyces cerevisiae for second-generation ethanol production from xylo-oligosaccharides and acetate.Scientific reports · 2023Article
- Bioethanol Production from Lignocellulosic Biomass-Challenges and Solutions.Molecules (Basel, Switzerland) · 2022Review
- Production of cellulosic ethanol and value-added products from corn fiber.Bioresources and bioprocessing · 2022Review
- Strategies for Efficient Expression of Heterologous Monosaccharide Transporters inJournal of fungi (Basel, Switzerland) · 2022Article
- Cellulosic Ethanol Production Using a Dual Functional Novel Yeast.International journal of microbiology · 2022Review
- Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- l-Lactic Acid Production Using EngineeredJournal of fungi (Basel, Switzerland) · 2021Article
- Transcriptomic Changes Induced by Deletion of Transcriptional RegulatorFrontiers in bioengineering and biotechnology · 2021Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylose, the second most abundant sugar in lignocellulosic biomass hydrolysates, can be fermented by Saccharomyces cerevisiae expressing one of two heterologous xylose pathways: a xylose oxidoreductase pathway and a xylose isomerase pathway. Depending on the type of the pathway, its optimization strategies and the fermentation efficiencies vary significantly. In the present study, we constructed two isogenic strains expressing either the oxidoreductase pathway (XYL123) or the isomerase pathway (XI-XYL3), and delved into simple and reproducible ways to improve the resulting strains. First, the strains were subjected to the deletion of PHO13, overexpression of TAL1, and adaptive evolution, but those individual approaches were only effective in the XYL123 strain but not in the XI-XYL3 strain. Among other optimization strategies of the XI-XYL3 strain, we found that increasing the copy number of the xylose isomerase gene (xylA) is the most promising but yet preliminary strategy for the improvement. These results suggest that the oxidoreductase pathway might provide a simpler metabolic engineering strategy than the isomerase pathway for the development of efficient xylose-fermenting strains under the conditions tested in the present study.
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