Evidence map›Paper›PMID 32715641›Full record

ArticleJournal of cellular and molecular medicine2020

CXXC finger protein 4 inhibits the CDK18-ERK1/2 axis to suppress the immune escape of gastric cancer cells with involvement of ELK1/MIR100HG pathway.

Ping Li, Dongfang Ge, Pengfei Li, Fangyong Hu, Junfeng Chu, Xiaojun Chen, Wenbo Song, Ali Wang, Guangyu Tian, Xiang Gu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 36 citations in OpenAlex.

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  8. A concise review on the role of MIR100HG in human disorders.Journal of cellular and molecular medicine · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Ping LiDepartment of Central Laboratory, Huaian Tumor Hospital & Huaian Hospital of Huaian City, Huaian, China.
Dongfang GeDepartment of Central Laboratory, Huaian Tumor Hospital & Huaian Hospital of Huaian City, Huaian, China.
Pengfei LiDepartment of Central Laboratory, Huaian Tumor Hospital & Huaian Hospital of Huaian City, Huaian, China.
Fangyong HuDepartment of Central Laboratory, Huaian Tumor Hospital & Huaian Hospital of Huaian City, Huaian, China.
Junfeng ChuDepartment of Oncology, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, China.
Xiaojun ChenDepartment of Oncology, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, China.
Wenbo SongDepartment of Oncology, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, China.
Ali WangDepartment of Oncology, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, China.
Guangyu TianDepartment of Oncology, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, China.
Xiang GuDepartment of Oncology, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, China.ORCID 0000-0003-0673-3654
Northern Jiangsu People's Hospital · CNHuaian First People’s Hospital · CNUniversity of Mannheim · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gastric cancer, is the fourth most common tumour type yet, ranks second in terms of the prevalence of cancer-related deaths worldwide. CXXC finger protein 4 (CXXC4) has been considered as a novel cancer suppressive factor, including gastric cancer. This study attempted to investigate the possible function of CXXC4 in gastric cancer and the underlying mechanism. The binding of the ETS domain-containing protein-1 (ELK1) to the long non-coding RNA MIR100HG promoter region was identified. Then, their expression patterns in gastric cancer tissues and cells (SGC7901) were detected. A CCK-8 assay was used to detect SGC7901 cell proliferation. Subsequently, SGC7901 cells were co-cultured with CD3+ T cells, followed by measurement of CD3+ T cell proliferation, magnitude of IFN-γ+ T cell population and IFN-γ secretion. A nude mouse model was subsequently developed for in vivo validation of the in vitro results. Low CXXC4 expression was found in SGC7901 cells. Nuclear entry of ELK1 can be inhibited by suppression of the extent of ELK1 phosphorylation. Furthermore, ELK1 is able to bind the MIR100HG promoter. Overexpression of CXXC4 resulted in weakened binding of ELK1 to the MIR100HG promoter, leading to a reduced proliferative potential of SGC7901 cells, and an increase in IFN-γ secretion from CD3+ T cells. Moreover, in vivo experiments revealed that CXXC4 inhibited immune escape of gastric cancer cells through the ERK1/2 axis. Inhibition of the CXXC4/ELK1/MIR100HG pathway suppressed the immune escape of gastric cancer cells, highlighting a possible therapeutic target for the treatment of gastric cancer.

Indexed as

AdultAgedAnimalsCD3 ComplexCell LineCell Line, TumorCell ProliferationCyclin-Dependent KinasesDNA-Binding Proteinsets-Domain Protein Elk-1FemaleHumansInterferon-gammaMaleMAP Kinase Signaling SystemMiceCD3 ComplexCXXC4 protein, humanCyclin-Dependent KinasesDNA-Binding ProteinsELK1 protein, humanets-Domain Protein Elk-1Interferon-gammaMicroRNAsPCTAIRE-3 protein kinaseTranscription FactorsCXXC finger protein 4ETS domain-containing protein-1gastric cancerimmune escapelong non-coding RNA MIR100HG

Identifiers

PMID32715641
PMCPMC7520267
OpenAlexW3045823499

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.