Evidence map›Paper›PMID 32715541›Full record

ArticleProtein science : a publication of the Protein Society2020

Structure-guided evolution of Green2 toward photostability and quantum yield enhancement by F145Y substitution.

Tingting Sun, Tianpeng Li, Ke Yi, Xiaolian Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tingting SunCollege of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang, Shandong, China.ORCID 0000-0003-3298-3075
Tianpeng LiCollege of City and Architecture Engineering, Zaozhuang University, Zaozhuang, Shandong, China.
Ke YiLaboratory of Medical Genetics, Central South University, Changsha, Hunan, China.
Xiaolian GaoDepartment of Biology and Biochemistry, University of Houston, Houston, Texas, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantum yield is a determinant for fluorescent protein (FP) applications and enhancing FP brightness through gene engineering is still a challenge. Green2, our de novo FP synthesized by microfluidic picoarray and cloning, has a significantly lower quantum yield than enhanced green FP, though they have high homology and share the same chromophore. To increase its quantum yield, we introduced an F145Y substitution into Green2 based on rational structural analysis. Y145 significantly increased the quantum yield (0.22 vs. 0.18) and improved the photostability (t

Indexed as

Amino Acid SubstitutionModels, MolecularMutation, MissenseGreen Fluorescent ProteinsProtein Stabilityenhanced green fluorescent proteinGreen Fluorescent ProteinsF145Y substitutionfluorescent proteinsphotostablilityquantum yieldsite-directed mutagenesis

Identifiers

PMID32715541
PMCPMC7454421

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.