ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2020
"Two Birds with One Stone" Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 75 citations in OpenAlex.
- Ruthenium Complexes in Protein Science: Heading a New Generation of Therapeutic and Analytical Tools.Angewandte Chemie (International ed. in English) · 2026Review
- Brightening New Horizons: Luminescent Transition Metal Complexes in Optical Imaging and Theranostic Applications.ACS central science · 2025Review
- Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications.Chemical reviews · 2024Review
- THQ-Xanthene: An Emerging Strategy to Create Next-Generation NIR-I/II Fluorophores.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Determination and Imaging of Small Biomolecules and Ions Using Ruthenium(II) Complex-Based Chemosensors.Topics in current chemistry (Cham) · 2022Review
- On-off-on fluorescent sensor for glutathione based on bifunctional vanadium oxide quantum dots induced spontaneous formation of MnOMikrochimica acta · 2021Article
- Ultrasensitive, Specific, and Rapid Fluorescence Turn-On Nitrite Sensor Enabled by Precisely Modulated Fluorophore Binding.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- "Two Birds with One Stone" Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, a "two birds with one stone" ruthenium(II) complex probe, Ru-NBD, is proposed as an effective tool for biothiols detection and discrimination in vitro and in vivo. Ru-NBD is nonluminescent due to the quenching of Ru(II) complex emission by photoinduced electron transfer (PET) from Ru(II) center to NBD and the quenching of NBD emission through 4-substitution with "O" ether bond. Ru-NBD is capable of reacting with Cys/Hcy to form long-lived red-emitting Ru-OH and short-lived green-emitting NBD-NR, while reacting with GSH to produce Ru-OH and nonemissive NBD-SR. The long lifetime emission of Ru(II) complex allows elimination of short lifetime background and NBD-NR fluorescence for total biothiols detection ("bird" one) by time-gated luminescence (TGL) analysis, and the remarkable difference in luminescence color response allows discrimination GSH and Cys/Hcy ("bird" two) through steady-state luminescence analysis. Ru-NBD features high sensitivity and selectivity, rapid luminescence response, and low cytotoxicity, which enables it to be used as the probe for luminescence and background-free TGL detection and visualization of biothiols in live cells, zebrafish, and mice. The successful development of this probe is anticipated to contribute to the future biological studies of biothiols roles in various diseases.
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