ArticleMethods in enzymology2020
Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG).
Article in Methods in enzymology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Beyond the Sequence: Chemical and Topological Design and Innovations in mRNA Therapeutics.Chemical reviews · 2026Review
- Efficient post-synthesis incorporation and conjugation of reactive ketones in RNAChemical communications (Cambridge, England) · 2023Article
- RNA-TAG Mediated Protein-RNA Conjugation.Chembiochem : a European journal of chemical biology · 2023Article
- Site-Specific Covalent Labeling of DNA Substrates by an RNA Transglycosylase.Journal of the American Chemical Society · 2023Article
- Site-Specific Fluorescent Labeling of RNA Interior Positions.Molecules (Basel, Switzerland) · 2021Review
- Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-Protein Interactions.ACS chemical biology · 2020Article
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Authors and funding
2 authors.
Funding
Abstract
Technologies for the labeling, detection, and manipulation of biomolecules have drastically improved our understanding of cell biology. As the myriad of functional roles for RNA in the cell are increasingly recognized, such tools to enable further investigation of RNA are the subject of much interest. RNA-TAG is an enzymatic method for site-specific, covalent labeling of RNA. This methodology makes use of a bacterial tRNA modifying enzyme, tRNA guanine transglycosylase, to incorporate modified substrate analogs into a target RNA, resulting in highly efficient and site-specific RNA labeling. In this chapter, we introduce the underlying principles of the RNA labeling reaction, discuss various applications of RNA-TAG, and present protocols for labeling specific RNA transcripts using this system.
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