ArticleMethods in enzymology2020
Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy.
Article in Methods in enzymology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice.NPJ science of food · 2024Article
- Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023Article
- Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (International ed. in English) · 2023Article
- Targetable Conformationally Restricted Cyanines Enable Photon-Count-Limited Applications*.Angewandte Chemie (International ed. in English) · 2021Article
- Single-cell omics analyses with single molecular detection: challenges and perspectives.Journal of biomedical research · 2021Article
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5 authors.
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Abstract
Pentamethine cyanines are a class of far-red fluorophores that find extensive use in single-molecule localization microscopy (SMLM), as well as a broad range of other techniques. A drawback of this scaffold is its relatively low quantum yields, which is due to excited state deactivation via trans-to-cis chromophore isomerization. Here we describe a synthetic strategy to improve the photon output of these molecules. In the key synthetic transformation, a protected dialdehyde precursor undergoes a cascade reaction that forms a tetracyclic ring system. The resulting conformationally restrained analogs exhibit improved fluorescence quantum yield and extended fluorescence lifetimes. These properties, together with their ability to efficiently recover from hydride reduction, enable a uniquely simple form of single-molecule localization microscopy (SMLM).
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