Evidence map›Paper›PMID 32712920›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

SHAPE Profiling to Probe Group II Intron Conformational Dynamics During Splicing.

Timothy Wiryaman, Navtej Toor

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Timothy WiryamanDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
Navtej ToorDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA. ntoor@ucsd.edu.

Funding

GENETIC MECHANISMS AND REGULATIONT32GM007240 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAMPTON, RANDOLPH Y. · 1985 to 2019
$23.8M
Structural Biology of Retrotransposition and pre-mRNA SplicingR35GM141706 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Navtej Singh Toor · 2021 to 2026
$2.6M
Structural Biology of Retrotransposition (Equipment Supplement)R01GM123275 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TOOR, NAVTEJ SINGH · 2017 to 2020
$1.3M
NIGMS NIH HHS R01 GM123275NIGMS NIH HHS R35 GM141706NIGMS NIH HHS T32 GM007240
6 · The paper itself

Abstract

Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) is a widely used technique for studying the structure and function of RNA molecules. It characterizes the flexibility of single nucleotides in the context of the local RNA structure. Here we describe the application of SHAPE-MaP (mutational profiling) to study different conformational states of the group II intron during the self-splicing reaction.

Indexed as

Nucleic Acid ConformationAcylationDNA Mutational AnalysisHigh-Throughput Nucleotide SequencingIntronsMutationReverse TranscriptionRNARNA SplicingSequence Analysis, RNASoftwareRNAChemical structure probingGroup II intronNext-generation sequencingRNA structureSHAPE-MaP

Identifiers

PMID32712920
PMCPMC7510887

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.