ArticleAnalytical and bioanalytical chemistry2020
Integrated silica membrane-based nucleic acid purification, amplification, and visualization platform for low-cost, rapid detection of foodborne pathogens.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Chitosan oligosaccharide lactate (COL) chosen from different derivatives protects DNA against environmental stressors.Analytical and bioanalytical chemistry · 2026Article
- Development and evaluation of a novel rapid fungal nucleic acid extraction kit.Archives of microbiology · 2025Article
- Establishment of rapid extraction and sensitive detection system of trace corn syrup DNA in honey.Food chemistry. Molecular sciences · 2024Article
- Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Multifunctional CRISPR-Cas12a.ACS applied materials & interfaces · 2024Article
- UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood.ACS applied materials & interfaces · 2023Article
- Rapid solid phase microextraction of DNA using mesoporous metal-organic framework coating for PCR-based identification of meat adulteration.Mikrochimica acta · 2022Article
- Recent developments and trends of automatic nucleic acid detection systems.Journal of biosafety and biosecurity · 2022Review
- Microfluidics-based strategies for molecular diagnostics of infectious diseases.Military Medical Research · 2022Review
- Comparison of paper-based nucleic acid extraction materials for point-of-care testing applications.Cellulose (London, England) · 2022Article
- Nucleic acid extraction without electrical equipment via magnetic nanoparticles in Pasteur pipettes for pathogen detection.Analytical biochemistry · 2021Article
- A simple methodology for RNA isolation from bacteria by integration of formamide extraction and chitosan-modified silica purification.Analytical and bioanalytical chemistry · 2021Article
- Entomo-virological surveillance of arboviruses in the Americas: prospects for the use of paper-based nucleic acid stabilization materials.Therapeutic advances in infectious diseaseReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Real-time fluorescence detection of nucleic acid exhibit excellent performance in analytical and diagnostic applications. However, the requirement of laboratory-based instrument and complex nucleic acid extraction greatly limits their application in point-of-care testing (POCT). Herein, a novel integrated silica membrane-based platform incorporating nucleic acid purification, amplification, and detection steps was developed. A universal and portable visualization platform was fabricated by incorporating denaturation bubble-mediated strand exchange amplification (SEA) reaction with silica membrane. The fluorescence signal of SYBR Green I with amplification products was visualized by the naked eye using a simple ultraviolet light on the silica membrane, and significant discrimination between the positive and negative samples could be easily and visually obtained. Besides, chitooligosaccharide-modified silica membrane allows the purification of nucleic acid in a totally aqueous system and enables in situ SEA. With the proposed integrated platform, 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.