Evidence map›Paper›PMID 32712466›Full record

ArticleiScience2020

Imaging Mass Spectrometry Reveals Tumor Metabolic Heterogeneity.

Yang Zhang, Christelle Guillermier, Thomas De Raedt, Andrew G Cox, Ophelia Maertens, Dean Yimlamai, Mingyue Lun, Adam Whitney, Richard L Maas, Wolfram Goessling and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Imaging plant metabolism in situ.Journal of experimental botany · 2024
    Review
  12. Article
  13. Promise of spatially resolved omics for tumor research.Journal of pharmaceutical analysis · 2023
    Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Mass spectrometry imaging of L-[ring-Cancer & metabolism · 2021
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yang ZhangDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Aging Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Christelle GuillermierDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Thomas De RaedtDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Andrew G CoxDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Ophelia MaertensDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Dean YimlamaiHarvard Medical School, Boston, MA, USA; Boston Children's Hospital, Boston, MA, USA.
Mingyue LunDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA.
Adam WhitneyDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA.
Richard L MaasDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Wolfram GoesslingDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Karen CichowskiDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Ludwig Center, Dana-Farber/Harvard Cancer Center, Boston, MA, USA.
Matthew L SteinhauserDepartment of Medicine, Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Aging Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address: msteinhauser@pitt.edu.

Funding

Metabolic Regulation of Liver GrowthR01DK105198 · NIDDK · BRIGHAM AND WOMEN'S HOSPITAL · PI GOESSLING, WOLFRAM · 2016 to 2020
$2.7M
A new modality to image tumor metabolic heterogeneity at subcellular resolutionDP2CA216362 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI STEINHAUSER, MATTHEW · 2016 to 2016
$2.7M
Deciphering Epithelial Signals in the Liver to Drive Inflammation and FibrosisR01DK129552 · NIDDK · YALE UNIVERSITY · PI YIMLAMAI, DEAN · 2021 to 2025
$2.2M
Hippo Signaling Mediates the Development of Liver FibrosisK08DK105351 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI YIMLAMAI, DEAN · 2015 to 2019
$796k
NCI NIH HHS DP2 CA216362NIDDK NIH HHS K08 DK105351NIDDK NIH HHS R01 DK105198NIDDK NIH HHS R01 DK129552
6 · The paper itself

Abstract

Malignant tumors exhibit high degrees of genomic heterogeneity at the cellular level, leading to the view that subpopulations of tumor cells drive growth and treatment resistance. To examine the degree to which tumors also exhibit metabolic heterogeneity at the level of individual cells, we employed multi-isotope imaging mass spectrometry (MIMS) to quantify utilization of stable isotopes of glucose and glutamine along with a label for cell division. Mouse models of melanoma and malignant peripheral nerve sheath tumors (MPNSTs) exhibited striking heterogeneity of substrate utilization, evident in both proliferating and non-proliferating cells. We identified a correlation between metabolic heterogeneity, proliferation, and therapeutic resistance. Heterogeneity in metabolic substrate usage as revealed by incorporation of glucose and glutamine tracers is thus a marker for tumor proliferation. Collectively, our data demonstrate that MIMS provides a powerful tool with which to dissect metabolic functions of individual cells within the native tumor environment.

Indexed as

Biological SciencesCancer Systems Biology

Identifiers

PMID32712466
PMCPMC7390776

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.