Evidence map›Paper›PMID 32711535›Full record

ArticleJournal of nanobiotechnology2020

Neuron-derived exosomes-transmitted miR-124-3p protect traumatically injured spinal cord by suppressing the activation of neurotoxic microglia and astrocytes.

Dongdong Jiang, Fangyi Gong, Xuhui Ge, Chengtang Lv, Chenyu Huang, Shuang Feng, Zheng Zhou, Yuluo Rong, Jiaxing Wang, Chengyue Ji and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 174 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
174citing papers in PubMed, 4 pooled it
11.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

174 citing papers in PubMed, 4 syntheses or guidelines pooled it, 300 citations in OpenAlex.

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114 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 1 country.

Dongdong JiangDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Fangyi GongDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Xuhui GeDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Chengtang LvDepartment of Orthopaedics, Yancheng Third People's Hospital, Yancheng, 224000, Jiangsu, China.
Chenyu HuangDepartment of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, Jiangsu, China.
Shuang FengDepartment of Encephalopathy, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210001, Jiangsu, China.
Zheng ZhouDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Yuluo RongDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Jiaxing WangDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Chengyue JiDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Jian ChenDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Wene ZhaoDepartment of Analytical & Testing Center, Nanjing Medical University, Nanjing, 211666, Jiangsu, China.
Jin FanDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China. fanjinnjmu@163.com.
Wei LiuDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China. liuweiorth@njmu.edu.cn.
Weihua CaiDepartment of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China. caiwhsubmission@sina.com.
Nanjing Medical University · CNJiangsu Province Hospital · CNNanjing University of Chinese Medicine · CNYancheng Third People's Hospital · CN

Funding

National Natural Science Foundation of China 81902211National Natural Science Foundation of China 81974335Natural Science Foundation of Jiangsu Province BK20181490Natural Science Foundation of Jiangsu Province BK20191061Six Talent Peaks Project in Jiangsu Province TD-SWYY-010
6 · The paper itself

Abstract

backgroundSpinal cord injury (SCI) is a catastrophic injury that can cause irreversible motor dysfunction with high disability. Exosomes participate in the transport of miRNAs and play an essential role in intercellular communication via transfer of genetic material. However, the miRNAs in exosomes which derived from neurons, and the underlying mechanisms by which they contribute to SCI remain unknown.

methodsA contusive in vivo SCI model and a series of in vitro experiments were carried out to explore the therapeutic effects of exosomes. Then, a miRNA microarray analysis and rescue experiments were performed to confirm the role of neuron-derived exosomal miRNA in SCI. Western blot, luciferase activity assay, and RNA-ChIP were used to investigate the underlying mechanisms.

resultsThe results indicated that neuron-derived exosomes promoted functional behavioral recovery by suppressing the activation of M1 microglia and A1 astrocytes in vivo and in vitro. A miRNA array showed miR-124-3p to be the most enriched in neuron-derived exosomes. MYH9 was identified as the target downstream gene of miR-124-3p. A series of experiments were used to confirm the miR-124-3p/MYH9 axis. Finally, it was found that PI3K/AKT/NF-κB signaling cascades may be involved in the modulation of microglia by exosomal miR-124-3p.

conclusionA combination of miRNAs and neuron-derived exosomes may be a promising, minimally invasive approach for the treatment of SCI.

Indexed as

MicroRNAsSpinal Cord InjuriesAnimalsAstrocytesCells, CulturedExosomesMaleMiceMice, Inbred C57BLMicrogliaMyosin Heavy ChainsNeuronsMicroRNAsMirn124 microRNA, mouseMyh9 protein, mouseMyosin Heavy ChainsAstrocytesExosomesMicrogliamiR-124-3p/MYH9 axisSpinal cord injury

Identifiers

PMID32711535
PMCPMC7382861
OpenAlexW3045238854

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.