ArticleBiophysical journal2020
Induction of Ordered Lipid Raft Domain Formation by Loss of Lipid Asymmetry.
Article in Biophysical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Effect of a scramblase activator upon lipid and probe scrambling and membrane domain formation in HEK 293T cells.Faraday discussions · 2025Article
- Lipid-driven interleaflet coupling of plasma membrane order regulates FcεRI signaling in mast cells.Biophysical journal · 2024Article
- Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging.Scientific reports · 2024Article
- Is cholesterol both the lock and key to abnormal transmembrane signals in Autism Spectrum Disorder?Lipids in health and disease · 2024Article
- Article
- Protein-lipid charge interactions control the folding of outer membrane proteins into asymmetric membranes.Nature chemistry · 2023Article
- Cholesterol, Eukaryotic Lipid Domains, and an Evolutionary Perspective of Transmembrane Signaling.Cold Spring Harbor perspectives in biology · 2023Review
- Dynamics of asymmetric membranes and interleaflet coupling as intermediates in membrane fusion.Biophysical journal · 2023Article
- Lipid packing is disrupted in copolymeric nanodiscs compared with intact membranes.Biophysical journal · 2023Article
- Mitocans induce lipid flip-flop and permeabilize the membrane to signal apoptosis.Biophysical journal · 2023Article
- Fluorescence Spectroscopic Analysis of Lateral and Transbilayer Fluidity of Exosome Membranes.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Lpcat3 deficiency promotes palmitic acid-induced 3T3-L1 mature adipocyte inflammation through enhanced ROS generation.Acta biochimica et biophysica Sinica · 2022Article
- Ordered Domain (Raft) Formation in Asymmetric Vesicles and Its Induction upon Loss of Lipid Asymmetry in Artificial and Natural Membranes.Membranes · 2022Article
- Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.The journal of physical chemistry. B · 2022Article
- Using cyclodextrin-induced lipid substitution to study membrane lipid and ordered membrane domain (raft) function in cells.Biochimica et biophysica acta. Biomembranes · 2022Review
- Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Loss of plasma membrane lipid asymmetry can induce ordered domain (raft) formation.Journal of lipid research · 2022Article
- Absence of Cardiolipin From the Outer Leaflet of a Mitochondrial Inner Membrane Mimic Restricts Opa1-Mediated Fusion.Frontiers in molecular biosciences · 2021Article
- Preparation and utility of asymmetric lipid vesicles for studies of perfringolysin O-lipid interactions.Methods in enzymology · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
How lipid asymmetry impacts ordered lipid domain (raft) formation may yield important clues to how ordered domain formation is regulated in vivo. Under some conditions, a sphingomyelin (SM) and cholesterol-rich ordered domain in one leaflet induces ordered domain formation in the corresponding region of an opposite leaflet composed of unsaturated phosphatidylcholine (PC) and cholesterol. In other conditions, the formation of ordered domains in a SM and cholesterol-rich leaflet can be suppressed by an opposite leaflet containing unsaturated PC and cholesterol. To explore how PC unsaturation influences the balance between these behaviors, domain formation was studied in asymmetric and symmetric lipid vesicles composed of egg SM, cholesterol, and either unsaturated dioleoyl PC (DOPC) or 1-palmitoyl 2-oleoyl PC (POPC). The temperature dependence of ordered domain formation was measured using Förster resonance energy transfer, which detects nanodomains as well as large domains. In cholesterol-containing asymmetric SM+PC outside/PC inside vesicles, the PC-containing inner leaflet tended to destabilize ordered domain formation in the SM+PC-containing outer leaflet relative to ordered domain stability in cholesterol-containing symmetric SM/PC vesicles. Residual ordered domain formation was detected in cholesterol-containing asymmetric SM+DOPC outside/DOPC inside vesicles, but ordered domain formation was completely or almost completely suppressed by asymmetry in cholesterol-containing SM+POPC outside/POPC inside vesicles over the entire temperature range measured. Suppression of ordered domain formation in the latter vesicles was confirmed by fluorescence anisotropy measurements. Because mixtures of SM, POPC, and cholesterol form domains in symmetric vesicles, and this lipid composition mimics plasma membranes to a significant degree, it is possible that under some conditions in vivo the loss of lipid asymmetry could trigger ordered domain formation.
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