ArticleThe Journal of infectious diseases2021
Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres.
Article in The Journal of infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Doxycycline Resistance and 16S rRNA Mutations in Treponema pallidum.Emerging infectious diseases · 2026Article
- Analysis ofmSphere · 2025Article
- Sequence variability of BamA and FadL candidate vaccinogens suggests divergent evolutionary paths ofJournal of bacteriology · 2025Article
- Article
- In vitro isolation of contemporary Treponema pallidum strains directly from clinical samples of syphilis patients.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2025Article
- Development and utilization ofmBio · 2025Article
- Clinical Presentation of Early Syphilis and Genomic Sequences of Treponema pallidum Strains in Patient Specimens and Isolates Obtained by Rabbit Inoculation.The Journal of infectious diseases · 2024Article
- Development and utilization ofbioRxiv : the preprint server for biology · 2024Article
- Turning the needle into the haystack: Culture-independent amplification of complex microbial genomes directly from their native environment.PLoS pathogens · 2024Review
- Majority of Treponema pallidum ssp. pallidum MLST allelic profiles in the Czech Republic (2004-2022) belong to two SS14-like clusters.Scientific reports · 2024Article
- Article
- Neurosyphilis: insights into its pathogenesis, susceptibility, diagnosis, treatment, and prevention.Frontiers in neurology · 2023Review
- Article
- Using Genomics to Understand the Epidemiology of Infectious Diseases in the Northern Territory of Australia.Tropical medicine and infectious disease · 2022Review
- A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.Nature communications · 2022Article
- Article
- High-throughput nanopore sequencing ofFrontiers in microbiology · 2022Article
- Global phylogeny of Treponema pallidum lineages reveals recent expansion and spread of contemporary syphilis.Nature microbiology · 2021Article
- Phylogenetic and genetic characterization of Treponema pallidum strains from syphilis patients in Japan by whole-genome sequence analysis from global perspectives.Scientific reports · 2021Article
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14 authors.
Funding
Abstract
backgroundWhole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples.
methodsWe built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China.
resultsBy combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions.
conclusionsThis approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
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