Evidence map›Paper›PMID 32710399›Full record

ArticleJournal of biomolecular NMR2020

Measuring transverse relaxation in highly paramagnetic systems.

Michele Invernici, Inês B Trindade, Francesca Cantini, Ricardo O Louro, Mario Piccioli

Abstract read
In one paragraph

Article in Journal of biomolecular NMR, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Paramagnetic NMR to study iron sulfur proteins:Journal of biomolecular NMR · 2023
    Article
  4. Studying Peptide-Metal Ion Complex Structures by Solution-State NMR.International journal of molecular sciences · 2022
    Review
  5. Article
  6. Article
  7. Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michele InverniciMagnetic Resonance Center (CERM) and Department of Chemistry, University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy.ORCID http://orcid.org/0000-0002-0312-7547
Inês B TrindadeInstituto de Tecnologia Química e Biológica António Xavier (ITQB-NOVA), Universidade Nova de Lisboa, Av. da República (EAN), 2780-157, Oeiras, Portugal.ORCID http://orcid.org/0000-0002-6746-8455
Francesca CantiniMagnetic Resonance Center (CERM) and Department of Chemistry, University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy.ORCID http://orcid.org/0000-0003-0526-6732
Ricardo O LouroInstituto de Tecnologia Química e Biológica António Xavier (ITQB-NOVA), Universidade Nova de Lisboa, Av. da República (EAN), 2780-157, Oeiras, Portugal. louro@itqb.unl.pt.ORCID http://orcid.org/0000-0002-2392-6450
Mario PiccioliMagnetic Resonance Center (CERM) and Department of Chemistry, University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, Italy. piccioli@cerm.unifi.it.ORCID http://orcid.org/0000-0001-9882-9754

Funding

Ente Cassa di Risparmio di Firenze 2016 0985European Cooperation in Science and Technology CA-15133Fundação para a Ciência e a Tecnologia LISBOA-01-0145-FEDER-007660H2020 Spreading Excellence and Widening Participation TWINNING TIMB3 810856Horizon 2020 Framework Programme ISTRUCT-ERIC 4509
6 · The paper itself

Abstract

The enhancement of nuclear relaxation rates due to the interaction with a paramagnetic center (known as Paramagnetic Relaxation Enhancement) is a powerful source of structural and dynamics information, widely used in structural biology. However, many signals affected by the hyperfine interaction relax faster than the evolution periods of common NMR experiments and therefore they are broadened beyond detection. This gives rise to a so-called blind sphere around the paramagnetic center, which is a major limitation in the use of PREs. Reducing the blind sphere is extremely important in paramagnetic metalloproteins. The identification, characterization, and proper structural restraining of the first coordination sphere of the metal ion(s) and its immediate neighboring regions is key to understand their biological function. The novel HSQC scheme we propose here, that we termed R

Indexed as

Magnetic Resonance SpectroscopyModels, MolecularNuclear Magnetic Resonance, BiomolecularAlgorithmsProtein ConformationProteinsProteinsIron sulfur proteinsNMR based structural restraintsParamagnetic NMRParamagnetic relaxation enhancementPulse sequencesTransverse relaxation

Identifiers

PMID32710399
PMCPMC7508935

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.