ArticleNature communications2020
Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Mitophagy coordinates organelle crosstalk in health and diseases.Signal transduction and targeted therapy · 2026Review
- Deep learning-enhanced single-shot triorganelle STED-FLIM imaging of lipid dynamics in living cells.Science advances · 2026Article
- Asymmetric Sulfhydryl-Substituted Squaraine Fluorophores for Sustained SIM Imaging in Live Cells.Journal of the American Chemical Society · 2026Article
- Organelle-specific fluorescent probes for live imaging at the nanoscale.Science China. Life sciences · 2026Review
- Spatial architecture of immunometabolism: Mitochondrial‑organelle interfaces in immune signaling (Review).International journal of molecular medicine · 2026Review
- Mitochondrial dynamics in neurodevelopment and neurodevelopmental disorders.Nature reviews. Neuroscience · 2026Review
- Cristae: bridging bioenergetic hubs and compartmental barriers in mitochondrial homeostasis.Cell death & disease · 2026Review
- Axial X-Ray Microscopy in Nanotomography.Tomography (Ann Arbor, Mich.) · 2026Article
- From Microscopy to Nanoscopy: Contemporary Physical Methods in Mitochondrial Structural Biology.International journal of molecular sciences · 2026Review
- High-content phenotyping reveals Golgi dynamics and their role in cell cycle regulation.The Journal of cell biology · 2026Article
- Advanced Imaging for Live-Cell Spatiotemporal Monitoring: Technologies and Applications.Research (Washington, D.C.) · 2026Review
- A bright and regenerative array tag with fluorogenic ligands for long-term live-cell nanoscopy and single-molecule tracking.Nature communications · 2025Article
- Topology-driven energy transfer networks for upconversion stimulated emission depletion microscopy.Light, science & applications · 2025Article
- Supramolecular Complexation of Quenched Rosamines with Cucurbit[7]Uril: Fluorescence Turn-ON Effect for Super-Resolution Imaging.Journal of the American Chemical Society · 2025Article
- Controllable Structure and Fluorescence Enhancement of ACQ Dye Nanoparticles Based on the FNP Process.Polymers · 2025Article
- A data-driven model for mitochondrial inner membrane remodeling as a driving force of organelle shaping.Journal of cell science · 2025Article
- Open-source 3D active sample stabilization for fluorescence microscopy.Biophysical reports · 2025Article
- Advances in research on mitochondrial dysfunction in neurodegenerative diseases.Journal of neurology · 2025Review
- HBimmCue: A Versatile Fluorescent Probe for Multi-Scale Imaging of Lipid Polarity and Membrane Order in Inner Mitochondrial Membrane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Fast segmentation and multiplexing imaging of organelles in live cells.Nature communications · 2025Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve to regulate their functionality. Conventional fluorescence microscopy can only provide ~300 nm resolution, which is insufficient to visualize mitochondrial cristae. Here, we developed an enhanced squaraine variant dye (MitoESq-635) to study the dynamic structures of mitochondrial cristae in live cells with a superresolution technique. The low saturation intensity and high photostability of MitoESq-635 make it ideal for long-term, high-resolution (stimulated emission depletion) STED nanoscopy. We performed time-lapse imaging of the mitochondrial inner membrane over 50 min (3.9 s per frame, with 71.5 s dark recovery) in living HeLa cells with a resolution of 35.2 nm. The forms of the cristae during mitochondrial fusion and fission can be clearly observed. Our study demonstrates the emerging capability of optical STED nanoscopy to investigate intracellular physiological processes with nanoscale resolution for an extended period of time.
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