Evidence map›Paper›PMID 32709382›Full record

ArticleFertility and sterility2020

Differential DNA methylation pattern and sperm quality in men with varicocele.

Viviane Paiva Santana, Emma Rae James, Cristiana Libardi Miranda-Furtado, Mateus Felipe de Souza, Camila Pinho Pompeu, Sandro Cassiano Esteves, Douglas T Carrell, Kenneth Ivan Aston, Timothy Gerald Jenkins, Rosana Maria Dos Reis

Abstract read
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In one paragraph

Article in Fertility and sterility, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 41 citations in OpenAlex.

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  20. Effects of Environmental and Pathological Hypoxia on Male Fertility.Frontiers in cell and developmental biology · 2021
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Viviane Paiva SantanaDepartment of Gynecology and Obstetrics, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
Emma Rae JamesDepartment of Surgery, School of Medicine, University of Utah, Salt Lake City, Utah.
Cristiana Libardi Miranda-FurtadoDepartment of Gynecology and Obstetrics, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, São Paulo, Brazil; Drug Research and Development Center, Postgraduate Program in Medical and Surgical Sciences, Federal University of Ceara, Fortaleza, Brazil.
Mateus Felipe de SouzaDepartment of Gynecology and Obstetrics, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
Camila Pinho PompeuANDROFERT, Andrology and Human Reproduction Clinic, Campinas, Brazil.
Sandro Cassiano EstevesANDROFERT, Andrology and Human Reproduction Clinic, Campinas, Brazil.
Douglas T CarrellDepartment of Surgery, School of Medicine, University of Utah, Salt Lake City, Utah.
Kenneth Ivan AstonDepartment of Surgery, School of Medicine, University of Utah, Salt Lake City, Utah.
Timothy Gerald JenkinsDepartment of Surgery, School of Medicine, University of Utah, Salt Lake City, Utah; Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah.
Rosana Maria Dos ReisDepartment of Gynecology and Obstetrics, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, São Paulo, Brazil. Electronic address: tim_jenkins@byu.edu.
Universidade de São Paulo · BRUniversity of Utah · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo study the global DNA methylation pattern in spermatozoa of patients with varicocele as well as investigate their semen quality.

designProspective observational case-control study.

settingUniversity-affiliated hospital. PATIENT(S): A total of 26 men with varicocele and 26 fertile men without the disorder.

interventionsAnalysis of semen quality and sperm DNA methylation patterns. MAIN OUTCOME MEASURE(S): Semen quality evaluated by semen analysis, and sperm DNA methylation patterns investigated using the Infinium MethylationEPIC BeadChip platform. RESULT(S): Men with varicocele displayed decreased semen quality. The sperm DNA methylation analysis showed that men with varicocele exhibit global hypomethylation in comparison with the control group. A total of 59 differentially methylated CpG sites were identified, most of them hypomethylated in the varicocele group. In regional analyses, 1,695 DNA regions were differentially methylated in men with varicocele. These regions show associations with gamete generation, meiotic and meiosis cell cycle, and semen quality based on gene ontology analysis. CONCLUSION(S): Gene ontology results suggest that changes in methylation may be associated with the low semen quality phenotype observed in some varicocele patients because the observed differentially methylated regions in varicocele patients are related to male reproductive pathways. Additionally, the varicocele grade may influence the magnitude of global sperm DNA methylation change. To our knowledge, this is the first report analyzing changes at a regional or CpG-specific level in men with varicocele.

Indexed as

AdultDNA MethylationHumansMaleSemen AnalysisSpermatozoaVaricoceleDNA methylationepigeneticssemen qualityspermatozoavaricocele

Identifiers

PMID32709382
OpenAlexW3045318684

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.