Evidence map›Paper›PMID 32706237›Full record

ArticleACS chemical biology2020

Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-Protein Interactions.

Kayla N Busby, Amitkumar Fulzele, Dongyang Zhang, Eric J Bennett, Neal K Devaraj

Abstract read
In one paragraph

Article in ACS chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Regulation of RNA-binding proteins by small biomolecules.Nature reviews. Molecular cell biology · 2026
    Review
  2. Article
  3. Article
  4. RNA-TAG Mediated Protein-RNA Conjugation.Chembiochem : a European journal of chemical biology · 2023
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. DNA Tiling Enables Precise Acylation-Based Labeling and Control of mRNA.Angewandte Chemie (International ed. in English) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kayla N BusbyDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.
Amitkumar FulzeleDivision of Biological Sciences, University of California, San Diego, La Jolla, California 92093, United States.
Dongyang ZhangDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.ORCID 0000-0003-2365-4763
Eric J BennettDivision of Biological Sciences, University of California, San Diego, La Jolla, California 92093, United States.
Neal K DevarajDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.ORCID 0000-0002-8033-9973

Funding

Enzymatic Site-Specific Labeling of RNA for Affinity IsolationR01GM123285 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DEVARAJ, NEAL KRISHNA · 2017 to 2020
$1.2M
Chemical Biology Interfaces at UC San DiegoT32GM112584 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BURKART, MICHAEL D. · 2015 to 2019
$729k
NIGMS NIH HHS R01 GM123285NIGMS NIH HHS T32 GM112584
6 · The paper itself

Abstract

Throughout their cellular lifetime, RNA transcripts are bound to proteins, playing crucial roles in RNA metabolism, trafficking, and function. Despite the importance of these interactions, identifying the proteins that interact with an RNA of interest in mammalian cells represents a major challenge in RNA biology. Leveraging the ability to site-specifically and covalently label an RNA of interest using

Indexed as

Base SequenceBiotinylationBlotting, NorthernBlotting, WesternChromatography, AffinityHeLa CellsHumansMutationNucleic Acid ConformationPentosyltransferasesProteinsProteomicsRNAPentosyltransferasesProteinsqueuine tRNA-ribosyltransferaseRNA

Identifiers

PMID32706237
PMCPMC10198264

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.