Evidence map›Paper›PMID 32705375›Full record

ArticleParasitology research2020

Development and characterization of 15 novel polymorphic microsatellite loci for two important bot flies (Diptera, Oestridae) by next-generation sequencing.

Gaël Aleix-Mata, Míriam Expósito, Francisco J Ruiz-Ruano, Ana María López-Beceiro, Luis E Fidalgo, Carlos Martínez-Carrasco, María Rocío Ruiz de Ybáñez, Mathieu Boos, Jesús M Pérez, Antonio Sánchez

Abstract read
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In one paragraph

Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Gaël Aleix-MataDepartment of Experimental Biology, Jaén University, E-23071, Jaén, Spain. galeix@ujaen.es.ORCID https://orcid.org/0000-0002-7429-4051
Míriam ExpósitoDepartment of Experimental Biology, Jaén University, E-23071, Jaén, Spain.
Francisco J Ruiz-RuanoDepartment of Genetics, Faculty of Sciences, Granada University, E-18071, Granada, Spain.ORCID https://orcid.org/0000-0002-5391-301X
Ana María López-BeceiroDepartment of Anatomy, Animal Production and Veterinary Clinical Sciences, University of Santiago de Compostela, E-27002, Lugo, Spain.
Luis E FidalgoDepartment of Anatomy, Animal Production and Veterinary Clinical Sciences, University of Santiago de Compostela, E-27002, Lugo, Spain.
Carlos Martínez-CarrascoDepartment of Animal Health, Murcia University, E-30100, Murcia, Spain.ORCID https://orcid.org/0000-0002-8742-0109
María Rocío Ruiz de YbáñezDepartment of Animal Health, Murcia University, E-30100, Murcia, Spain.
Mathieu BoosResearch Agency in Applied Ecology, Naturaconst@, 67270, Wilshausen, France.
Jesús M PérezDepartment of Animal and Plant Biology and Ecology, Jaén University, E-23071, Jaén, Spain.ORCID https://orcid.org/0000-0001-9159-0365
Antonio SánchezDepartment of Experimental Biology, Jaén University, E-23071, Jaén, Spain. abaca@ujaen.es.ORCID https://orcid.org/0000-0002-6715-8158
Universidad de Jaén · ESUniversidad de Murcia · ESUniversidade de Santiago de Compostela · ESUppsala University · SE

Funding

Fédération Nationale des Chasseurs (France) FNC-PSN-PR5-2013
6 · The paper itself

Abstract

Cephenemyia stimulator and Oestrus ovis are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies.

Indexed as

AllelesAnimalsDipteraGenetics, PopulationHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsMyiasisPolymerase Chain ReactionSheepCephenemyia stimulatorMicrosatellitesNext-generation sequencingOestrus ovis

Identifiers

PMID32705375
OpenAlexW3044602010

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.