Evidence map›Paper›PMID 32705190›Full record

ArticleMolecular medicine reports2020

Influence of reaming intramedullary nailing on MSC population after surgical treatment of patients with long bone fracture.

Ioannis Sperelakis, Eliza Tsitoura, Chara Koutoulaki, Semeli Mastrodimou, Theodoros H Tosounidis, Demetrios A Spandidos, Katerina M Antoniou, George Kontakis

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Ioannis SperelakisDepartment of Orthopedics and Traumatology, University of Crete School of Medicine, 71003 Heraklion, Greece.
Eliza TsitouraDepartment of Respiratory Medicine, University General Hospital of Heraklion, Laboratory of Molecular and Cellular Pneumonology, Medical School, University of Crete, 71003 Heraklion, Greece.
Chara KoutoulakiDepartment of Respiratory Medicine, University General Hospital of Heraklion, Laboratory of Molecular and Cellular Pneumonology, Medical School, University of Crete, 71003 Heraklion, Greece.
Semeli MastrodimouDepartment of Respiratory Medicine, University General Hospital of Heraklion, Laboratory of Molecular and Cellular Pneumonology, Medical School, University of Crete, 71003 Heraklion, Greece.
Theodoros H TosounidisDepartment of Orthopedics and Traumatology, University of Crete School of Medicine, 71003 Heraklion, Greece.
Demetrios A SpandidosLaboratory of Clinical Virology, Medical School, University of Crete, 71003 Heraklion, Greece.
Katerina M AntoniouDepartment of Respiratory Medicine, University General Hospital of Heraklion, Laboratory of Molecular and Cellular Pneumonology, Medical School, University of Crete, 71003 Heraklion, Greece.
George KontakisDepartment of Orthopedics and Traumatology, University of Crete School of Medicine, 71003 Heraklion, Greece.
University of Crete · GRUniversity Hospital of Heraklion · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reamed intramedullary nailing (RIN) is a surgical method of choice for treatment of diaphyseal fractures. This procedure affects the biological environment of bone tissue locally and systemically. This study investigated the influence of RIN on mesenchymal stem cells (MSCs) in patients with long bone fractures. The axis of C-X-C motif chemokine receptor 4 (CXCR4)/stromal cell-derived factor 1 (SDF‑1) was selected since it is considered as major pathway for MSC homing and migration. Iliac crest bone marrow (IC‑BM) samples and blood samples were collected at two different time points. One sample was collected before the RIN (BN) and the other immediately after RIN (AN). BM‑MSCs were cultured and RT‑qPCR was performed for CXCR4 mRNA levels and ELISA for the SDF‑1 sera levels. The experimental study revealed that there was a correlation between the increase of SDF‑1 levels in peripheral blood and a decrease in the levels of CXCR4 in MSCs in the IC‑BM following RIN. The levels of SDF‑1 showed a significant increase in the sera of patients after RIN. In conclusion, the present study is the first providing evidence of the effects of RIN on MSC population via the CXCR4/SDF‑1 axis. The levels of serum SDF‑1 factor were elevated after RIN while increased levels of SDF‑1 in peripheral blood were inversely correlated with the mRNA levels of CXCR4 on BM‑MSCs after RIN. Therefore, this study contributes to enlighten the systematic effects of RIN on the population of MSCs at a cellular level.

Indexed as

AdultCells, CulturedChemokine CXCL12FemaleFracture Fixation, IntramedullaryFractures, BoneHumansMaleMesenchymal Stem CellsMiddle AgedReceptors, CXCR4Up-RegulationYoung AdultChemokine CXCL12CXCL12 protein, humanCXCR4 protein, humanReceptors, CXCR4cXcr4long bone fracturemesenchymal stem cellsreaming intramedullary nailingSdF-1

Identifiers

PMID32705190
PMCPMC7411410
OpenAlexW3041088899

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.