ArticleTranslational vision science & technology2020
Visualization of the Retina in Intact Eyes of Mice and Ferrets Using a Tissue Clearing Method.
Article in Translational vision science & technology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Chronic dental lighting disrupts blood-retinal barrier homeostasis via vascular and inflammatory pathways.International journal of oral science · 2026Article
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- Article
- 3D visualization of cellular and molecular distributions in human crystalline lenses at different ages.American journal of translational research · 2024Article
- Light sheet fluorescence microscopy of cleared human eyes.Communications biology · 2023Article
- Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing.Communications biology · 2022Article
- Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice.Cells · 2022Article
- Morphology and Histology of the Orbital Region and Eye of the Asiatic Black Bear (Animals : an open access journal from MDPI · 2022Article
- Comparison between sodium iodate and lipid peroxide murine models of age-related macular degeneration for drug evaluation-a narrative review.Annals of eye science · 2022Article
- InVision: An optimized tissue clearing approach for three-dimensional imaging and analysis of intact rodent eyes.iScience · 2021Article
- Non-separate Mouse Sclerochoroid/RPE/Retina Staining and Whole Mount for the Integral Observation of Subretinal Layer.Bio-protocol · 2021Article
- Impact of aging on anterior segment morphology and aqueous humor dynamics in human Eyes: Advanced imaging and computational techniques.Biocybernetics and biomedical engineeringArticle
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: Visualization of specific cells and structures in intact organs would greatly facilitate our knowledge about pathological changes; therefore, a tissue clearing method applicable to the intact eye may be valuable. Here we report a novel imaging method for the retina using the hyperhydration-based tissue clearing technique CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational Analysis). Methods: Eyes of Institute of Cancer Research (ICR) mice, C57BL/6 mice, and normally pigmented sable ferrets ( Results: The combination of melanin bleaching and CUBIC efficiently made the eyes of C57BL/6 mice transparent. By combining melanin bleaching, CUBIC, and immunostaining, we succeeded in visualization of retinal structures from the outside of the intact eyes of mice. Furthermore, we found that our methods were applicable not only to mouse eyes but also to ferret eyes, which are much larger than those of mice. Conclusions: Our method was useful for visualizing specific cells and structures in the retina of intact eyes with single-cell resolution without making tissue sections. Translational Relevance: This simple and efficient method can be applicable to various rodent models, including those associated with glaucoma or myopia, and will facilitate evaluating the effects of novel therapy for relevant eye diseases by visualizing changes from the retina to the sclera at both molecular and macroscopic levels simultaneously in a whole-eye preparation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.