ArticleScientific reports2020
Effects of bacterial lipopolysaccharides on platelet function: inhibition of weak platelet activation.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Pathogenic strains of a gut commensal drive systemic platelet activation and thromboinflammation in lupus nephritis.bioRxiv : the preprint server for biology · 2025Article
- Functional toll-like receptor 4 links endotoxin sensing to platelet priming in feline platelets.Frontiers in veterinary science · 2025Article
- The differential formation and composition of leukocyte-platelet aggregates induced by various cellular stimulants.Thrombosis research · 2024Article
- Dynamics of platelet parameters in children with severe community-acquired pneumonia between viral and bacterial infections.Translational pediatrics · 2024Article
- Gastrodin Attenuates Colitis and Prevents Tumorigenesis in Mice by Interrupting TLR4/MD2/NF-κB Signaling Transduction.Anti-cancer agents in medicinal chemistry · 2024Article
- Nicotine's impact on platelet function: insights into hemostasis mechanisms.Frontiers in pharmacology · 2024Article
- Portal Vein Thrombosis in Patients with Liver Cirrhosis: What Went Wrong?International journal of general medicine · 2023Article
- Lentinan and β-glucan extract from shiitake mushroom,Iranian journal of basic medical sciences · 2023Article
- Limited value of coagulation parameters in diagnosing periprosthetic joint infection.International orthopaedics · 2022Article
- The Controversial Role of LPS in Platelet Activation In Vitro.International journal of molecular sciences · 2022Review
- Ex vivo observation of granulocyte activity during thrombus formation.BMC biology · 2022Article
- Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood.International journal of molecular sciences · 2021Article
- Pathological and Therapeutic Approach to Endotoxin-Secreting Bacteria Involved in Periodontal Disease.Toxins · 2021Review
- Proteomics: A Tool to Study Platelet Function.International journal of molecular sciences · 2021Review
- The Toll-Like Receptor 2 Ligand Pam2CSK4 Activates Platelet Nuclear Factor-κB and Bruton's Tyrosine Kinase Signaling to Promote Platelet-Endothelial Cell Interactions.Frontiers in immunology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Platelets are anucleate blood cells with reported roles in hemostasis and immune responses, which possess a functional receptor for bacterial lipopolysaccharides (LPSs), the well-known inducers of inflammation. However, LPSs effects on platelets are contradictory. Here we aim to investigate mechanisms of platelet functioning in the presence of LPS and to find the cause of the discrepancy in the previously published data. Cell activity was analyzed by flow cytometry, western blotting, and aggregometry. Thrombus growth was assessed by fluorescent microscopy. LPS' activity was checked by their capability to induce PMN activation. However, LPSs did not substantially affect either thrombus growth in flow chambers, irreversible platelet aggregation, or platelet responses to strong activation. Platelet aggregation in response to 1 μM of ADP was significantly inhibited by LPSs. Flow cytometry analysis revealed that platelet activation responses to weak stimulation were also diminished by LPSs, while VASP phosphorylation was weakly increased. Additionally, LPSs were capable of inhibition of ADP-induced P2-receptor desensitization. Incubation of platelets with a pan-PDE inhibitor IBMX significantly enhanced the LPSs-induced platelet inhibition, implying cAMP/cGMP dependent mechanism. The discrepancy in the previously published data could be explained by LPS-induced weak inhibition of platelet activation and the prevention of platelet desensitization.
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Registered trials
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