ArticleScientific reports2020
Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Short report: surveillance of hrp2/hrp3 deletions in Plasmodium falciparum within Haiti, 2021-2023, using RDTs.Malaria journal · 2026Article
- Current practices and innovative diagnostics for Malaria in Ethiopia: a scoping review.BMC infectious diseases · 2026Article
- Rapid diagnostic test kit as a source of DNA in comparison with filter paper for malaria molecular surveillance and drug resistance monitoring: a cross-sectional study.Malaria journal · 2026Article
- Integrating local malaria molecular monitoring into regular malaria indicator surveys on Bioko Island: high association between urban communities and low-density infections.Malaria journal · 2025Article
- Molecular Analysis of Rabies Virus Using RNA Extracted from Used Lateral Flow Devices.Journal of clinical microbiology · 2023Article
- Development and evaluation of PlasmoPod: A cartridge-based nucleic acid amplification test for rapid malaria diagnosis and surveillance.PLOS global public health · 2023Article
- Analysis of nucleic acids extracted from rapid diagnostic tests reveals a significant proportion of false positive test results associated with recent malaria treatment.Malaria journal · 2022Article
- Characterising co-infections with Plasmodium spp., Mansonella perstans or Loa loa in asymptomatic children, adults and elderly people living on Bioko Island using nucleic acids extracted from malaria rapid diagnostic tests.PLoS neglected tropical diseases · 2022Article
- An Optimized Real-Time qPCR Method for the Effective Detection of Human Malaria Infections.Diagnostics (Basel, Switzerland) · 2021Article
- Stratifying malaria receptivity in Bangladesh using archived rapid diagnostic tests.Malaria journal · 2020Article
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Authors and funding
23 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of malaria rapid diagnostic tests (RDTs) as a source for nucleic acids that can be analyzed via nucleic acid amplification techniques has several advantages, including minimal amounts of blood, sample collection, simplified storage and shipping conditions at room temperature. We have systematically developed and extensively evaluated a procedure to extract total nucleic acids from used malaria RDTs. The co-extraction of DNA and RNA molecules from small volumes of dried blood retained on the RDTs allows detection and quantification of P. falciparum parasites from asymptomatic patients with parasite densities as low as 1 Pf/µL blood using reverse transcription quantitative PCR. Based on the extraction protocol we have developed the ENAR (Extraction of Nucleic Acids from RDTs) approach; a complete workflow for large-scale molecular malaria surveillance. Using RDTs collected during a malaria indicator survey we demonstrated that ENAR provides a powerful tool to analyze nucleic acids from thousands of RDTs in a standardized and high-throughput manner. We found several, known and new, non-synonymous single nucleotide polymorphisms in the propeller region of the kelch 13 gene among isolates circulating on Bioko Island, Equatorial Guinea.
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