ArticleeLife2020
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
31 citing papers in PubMed.
- Volumetric DNA microscopy for mapping spatial transcriptomes in three dimensions.Nature protocols · 2026Review
- Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq.Nature protocols · 2026Review
- Single-strand deaminase-assisted editing for functional RNA manipulation.Nature biotechnology · 2026Article
- Rapid clinical validation of an RNA/DNA hybrid tagmentation-based metagenomic workflow for respiratory RNA virus detection.Frontiers in microbiology · 2026Article
- Single-cell epigenomics and proteomics methods integrated in multiomics.Fundamental research · 2025Review
- Methanol fixation and tagmentation of RNA/DNA hybrids directly enable single-cell transcriptome sequencing.Frontiers in genetics · 2025Article
- QUIC-seq: Quick ultra-affordable high-throughput convenient RNA sequencing.Plant biotechnology journal · 2025Article
- Near-cognate tRNAs increase the efficiency and precision of pseudouridine-mediated readthrough of premature termination codons.Nature biotechnology · 2025Article
- HIPSD&R-seq enables scalable genomic copy number and transcriptome profiling.Genome biology · 2024Article
- Global coupling of R-loop dynamics with RNA polymerase II modulates gene expression and early development of Drosophila.Nucleic acids research · 2024Article
- OpenTn5: Open-Source Resource for Robust and Scalable Tn5 Transposase Purification and Characterization.bioRxiv : the preprint server for biology · 2024Article
- Transposase-assisted tagmentation: an economical and scalable strategy for single-worm whole-genome sequencing.G3 (Bethesda, Md.) · 2024Article
- Transposase-Assisted RNA/DNA Hybrid Co-Tagmentation for Target Meta-Virome of Foodborne Viruses.Viruses · 2024Article
- Cost and time-efficient construction of a 3'-end mRNA library from unpurified bulk RNA in a single tube.Experimental & molecular medicine · 2024Article
- Selection of Flax Genotypes for Pan-Genomic Studies by Sequencing Tagmentation-Based Transcriptome Libraries.Plants (Basel, Switzerland) · 2023Article
- Single-nucleus multiomic mapping of mMolecular cell · 2023Article
- Surveying the global landscape of post-transcriptional regulators.Nature structural & molecular biology · 2023Article
- TRACE-seq: Rapid, Low-Input, One-Tube RNA-seq Library Construction Based on Tagmentation of RNA/DNA Hybrids.Current protocols · 2023Article
- Single-Cell Molecular Barcoding to Decode Multimodal Information Defining Cell States.Molecules and cells · 2023Review
- Transcriptome and DNA Methylome Analysis of Two Contrasting Rice Genotypes under Salt Stress during Germination.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro. As a proof-of-concept application, we utilized this activity to replace the traditional library construction procedure of RNA sequencing, which contains many laborious and time-consuming processes. Results of
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.