Evidence map›Paper›PMID 32700750›Full record

ArticleMolecular medicine reports2020

Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells.

Chenglong Li, Lu Yang, Xiaohua Ren, Mu Lin, Daonan Shen, You Li, Xiangyu Zhang, Chunhui Liu, Yandong Mu

RetractedOpen access · hybridAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Chenglong LiDepartment of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China.
Lu YangSchool of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, P.R. China.
Xiaohua RenDepartment of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China.
Mu LinDepartment of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China.
Daonan ShenWest China School and Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610063, P.R. China.
You LiWest China School and Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610063, P.R. China.
Xiangyu ZhangDepartment of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan 610041, P.R. China.
Chunhui LiuDepartment of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China.
Yandong MuDepartment of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China.
University of Electronic Science and Technology of China · CNSichuan University · CNSichuan Cancer Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hydroxyapatite scaffolds (HASs) are widely studied as suitable materials for bone replacement scaffolds due to their chemical similarities to organic materials. In our previous study, a novel HAS with a 25‑30‑µm groove structure (HAS‑G) exhibited enhanced osteogenesis of bone mesenchymal stromal cells (BMSCs) compared with HAS, potentially by modulating the macrophage‑induced immune microenvironment. However, the exact effects of different surface patterns on the physiological processes of attached cells is not known. The present study aimed to determine the effects of HAS‑G on the osteogenesis and physiological processes in BMSCs. Cell counting kit‑8 assays and propidium iodide staining followed by flow cytometry were performed, and the results demonstrated that both in normal medium and differentiating medium, HAS‑G promoted cell proliferation by decreasing the proportion of G1/G0 cells and decreased reactive oxygen species (ROS) accumulation in BMSCs compared with HAS. Detection markers of osteogenesis revealed that compared with HAS, HAS‑G increased runt‑related transcription factor 2, osteocalcin and osteopontin protein levels and promoted osteogenesis, which was further confirmed by Alizarin Red S staining. Following JC‑1 staining, it was observed that HAS‑G maintained the mitochondrial membrane potential, similar to that achieved by N‑acetylcysteine pretreatment. In addition, compared with those of HAS, HAS‑G decreased mitochondrial ROS levels, which potentially contributed to the promotion of osteogenesis. The results also demonstrated that HAS‑G inhibited mitophagy induced by ROS accumulation and ATP synthesis compared with HAS. In conclusion, HAS‑G decreased ROS accumulation and mitophagy and thus promoted osteogenesis of BMSCs, indicating that ROS modulation of HAS‑G may serve a key role in osteogenesis.

Indexed as

AnimalsBone MarrowCell CycleCell ProliferationCells, CulturedCore Binding Factor Alpha 1 SubunitDurapatiteFemaleHomeostasisMembrane Potential, MitochondrialMesenchymal Stem CellsMicroscopy, Electron, ScanningMitochondriaMitophagyOsteocalcinOsteogenesisCore Binding Factor Alpha 1 SubunitDurapatiteOsteocalcinOsteopontinReactive Oxygen SpeciesRUNX2 protein, humanbone mesenchymal stromal cellshydroxyapatite scaffoldmitochondrial homeostasismitophagyosteogenesis

Identifiers

PMID32700750
PMCPMC7453552
OpenAlexW3043907223

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.