Evidence map›Paper›PMID 32700263›Full record

ArticleJournal, genetic engineering & biotechnology2020

Complete genome sequence and comparative analysis of two potential probiotics Bacillus subtilis isolated from honey and honeybee microbiomes.

Abdelhamid A Hamdy, Mona A Esawy, Nouran A Elattal, Magdy A Amin, Amal E Ali, Ghada E A Awad, Ian Connerton, Nahla M Mansour

Open access · diamondAbstract read
In one paragraph

Article in Journal, genetic engineering & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

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  5. Reference-based read clustering improves theComputational and structural biotechnology journal · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Abdelhamid A HamdyChemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Div., National Research Centre, 33 El Bohouth St., Dokki, P.O. Box: 12622, Cairo, Egypt.
Mona A EsawyChemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Div., National Research Centre, 33 El Bohouth St., Dokki, P.O. Box: 12622, Cairo, Egypt.
Nouran A ElattalChemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Div., National Research Centre, 33 El Bohouth St., Dokki, P.O. Box: 12622, Cairo, Egypt.
Magdy A AminDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Amal E AliDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Ghada E A AwadChemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Div., National Research Centre, 33 El Bohouth St., Dokki, P.O. Box: 12622, Cairo, Egypt.
Ian ConnertonDivision of Food Sciences, School of Biosciences, University of Nottingham Loughborough, Sutton Bonington Campus, Leicestershire, LE12 5RD, UK.
Nahla M MansourChemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Div., National Research Centre, 33 El Bohouth St., Dokki, P.O. Box: 12622, Cairo, Egypt. nahla_mansour@hotmail.com.ORCID http://orcid.org/0000-0002-8045-7531
National Research Centre · EGCairo University · EGUniversity of Nottingham · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWe have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics. Here we report their complete genome sequence and comparative analysis with other and other B. subtilis strains.

resultsThe complete genome sequences of Bacillus subtilis HMNig-2 and MENO2 were de novo assembled from MiSeq paired-end sequence reads and annotated using the RAST tool. Whole-genome alignments were performed to identify functional differences associated with their potential use as probiotics.

conclusionsThe comparative analysis and the availability of the genome sequence of these two strains will provide comprehensive analysis about the diversity of these valuable Bacillus strains and the possible impact of the environment on bacterial evolution. SIGNIFICANCE AND IMPACT OF STUDY: We introduce complete genome sequence of two new B. subtilis strains HMNig-2 and MENO2 with probiotic potential and as cell factories for the production of levan and other valuable components for pharmaceutical and industrial applications.

Indexed as

Bacillus subtilisGenome sequenceHoneyHoneybeeProbiotics

Identifiers

PMID32700263
PMCPMC7376800
OpenAlexW3044429393

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.