ArticleArchives of toxicology2020
A novel human pluripotent stem cell-based assay to predict developmental toxicity.
Article in Archives of toxicology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Micropatterned neural induction with heat-inactivated extracellular matrix protein by on-demand high-speed laser.Regenerative therapy · 2026Article
- Embryotoxicity of pyrethroid insecticides and their mixtures in a human induced pluripotent stem cell-based model in vitro.Archives of toxicology · 2026Article
- Review
- In Vitro Bioassay Evidence for Chemical Mixture Propagation from the Environment to Humans.Environmental science & technology · 2026Article
- Embryotoxicity and mixture effects of legacy PFAS in a human iPSC-based 3D model.Cell biology and toxicology · 2026Article
- Advances in Cytotoxicity Testing: From In Vitro Assays to In Silico Models.International journal of molecular sciences · 2025Review
- Morphometric-Assisted Prediction of Developmental Toxicity Using Stem Cell-Based Embryo Models in Microwells.Advanced healthcare materials · 2025Article
- Implementing a trilineage differentiation in the ReproTracker assay for improved teratogenicity assessment.Frontiers in toxicology · 2025Article
- Embryoid Body Test: A Simple and Reliable Alternative Developmental Toxicity Test.International journal of molecular sciences · 2024Review
- Standardization and optimization of the hiPSC-based PluriLum assay for detection of embryonic and developmental toxicants.Archives of toxicology · 2024Article
- Investigating the applicability domain of the hiPSC-based PluriLum assay: an embryotoxicity assessment of chemicals and drugs.Archives of toxicology · 2024Article
- The ENDOMIX project: an interdisciplinary approach to understanding how real-life chemical mixtures target the immune system to trigger disease.Open research Europe · 2024Article
- Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Developmental Toxicity Studies: The Path towards Humanized 3D Stem Cell-Based Models.International journal of molecular sciences · 2023Review
- 2,4-di-tert-butylphenol exposure impairs osteogenic differentiation.Toxicology and applied pharmacology · 2023Article
- Human-Based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State of the Art with a Feto-Placental Organ-on-Chip Platform.International journal of environmental research and public health · 2022Review
- The Human Induced Pluripotent Stem Cell Test as an Alternative Method for Embryotoxicity Testing.International journal of molecular sciences · 2022Article
- Cell-Based Antioxidant Properties and Synergistic Effects of Natural Plant and Algal Extracts Pre and Post Intestinal Barrier Transport.Antioxidants (Basel, Switzerland) · 2022Article
- High Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Dictyostelium discoideum: An Alternative Nonanimal Model for Developmental Toxicity Testing.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a great need for novel in vitro methods to predict human developmental toxicity to comply with the 3R principles and to improve human safety. Human-induced pluripotent stem cells (hiPSC) are ideal for the development of such methods, because they are easy to retrieve by conversion of adult somatic cells and can differentiate into most cell types of the body. Advanced three-dimensional (3D) cultures of these cells, so-called embryoid bodies (EBs), moreover mimic the early developing embryo. We took advantage of this to develop a novel human toxicity assay to predict chemically induced developmental toxicity, which we termed the PluriBeat assay. We employed three different hiPSC lines from male and female donors and a robust microtiter plate-based method to produce EBs. We differentiated the cells into cardiomyocytes and introduced a scoring system for a quantitative readout of the assay-cardiomyocyte contractions in the EBs observed on day 7. Finally, we tested the three compounds thalidomide (2.3-36 µM), valproic acid (25-300 µM), and epoxiconazole (1.3-20 µM) on beating and size of the EBs. We were able to detect the human-specific teratogenicity of thalidomide and found the rodent toxicant epoxiconazole as more potent than thalidomide in our assay. We conclude that the PluriBeat assay is a novel method for predicting chemicals' adverse effects on embryonic development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.