ArticleScientific reports2020
Stress and behavioral correlates in the head-fixed method: stress measurements, habituation dynamics, locomotion, and motor-skill learning in mice.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 91 citations in OpenAlex.
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- A transparent wheel-based platform for locomotion-on-demand and multi-view body and facial kinematics in head-fixed mice.Scientific reports · 2026Article
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- Longitudinal Awake Mouse fMRI During Voluntary Locomotion Using Zero TE Imaging and a Novel Treadmill Training Protocol.Magnetic resonance in medicine · 2026Article
- GluK1 kainate receptors in parvalbumin interneurons modulate cortico-hippocampal network dynamics during social behavior.Translational psychiatry · 2026Article
- Revealing abrupt transitions from goal-directed to habitual behavior.Nature communications · 2026Article
- Opioid-specific brain connectivity dynamics distinguish analgesia from secondary effects: Studies in male mice.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Open-Source Platform for Adjustable Training Regimes in Freely Moving and Head-Fixed Mice.eNeuro · 2026Article
- Secondary Somatosensory Cortex Is Required for Learning but Not Execution of a Tactile Discrimination.The European journal of neuroscience · 2026Article
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- Adolescent stress exposure induces persistent, sex-specific cognitive deficits.Neurobiology of learning and memory · 2025Article
- Disruption of brain rhythms in a pharmacological model of schizophrenia in male and female mice.Biology of sex differences · 2025Article
- Longitudinal, simultaneous wide-field fluorescent Ca2+ imaging and fMRI in awake mice.bioRxiv : the preprint server for biology · 2025Article
- Training Mice to Perform Attentional Set-Shifting Under Head Restraint.Bio-protocol · 2025Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Manual restriction of head movement, or head-fixation, of awake rodents allows for sophisticated investigation of neural circuits in vivo, that would otherwise be impossible in completely freely moving animals. While it is known that head-fixation induces stress, the scale of this stress and habituation dynamics remain unclear. We used the Mobile HomeCage system (Neurotar Ltd, Finland) where animals have their heads fixed to an aluminum frame but are otherwise freely moving in an ultralight carbon container floating above an air-dispensing base. For 25 consecutive days, mice were head-fixed while standing on the air-lifted platform for 2 h per day and blood samples were taken periodically to measure variation in the stress-related hormone, corticosterone. We showed that the initial increase in corticosterone concentration is followed by a return to control level throughout the days of head-fixed training. We also found a locomotor correlate of this drop. We conducted a battery of stress-sensitive behavioral paradigms in freely-moving mice that revealed minor differences following chronic head-fixation. Finally, we analyzed motor-skill learning in the head-fixed setup with a floating container. We believe that our results may contribute to better interpretation of past literature and future in vivo experiments using head-fixed animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.