Evidence map›Paper›PMID 32699093›Full record

ArticleJournal of virology2020

A Hyperactive Kunjin Virus NS3 Helicase Mutant Demonstrates Increased Dissemination and Mortality in Mosquitoes.

Kelly E Du Pont, Nicole R Sexton, Martin McCullagh, Gregory D Ebel, Brian J Geiss

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Conserved motifs in the flavivirus NS3 RNA helicase enzyme.Wiley interdisciplinary reviews. RNA · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Kelly E Du Pont *Department of Chemistry, Colorado State University, Fort Collins, Colorado, USA.
Nicole R Sexton *Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
Martin McCullaghDepartment of Chemistry, Oklahoma State University, Stillwater, Oklahoma, USA.
Gregory D EbelArthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
Brian J GeissArthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA Brian.Geiss@colostate.edu.ORCID 0000-0001-8180-5772
Colorado State University · USOklahoma State University · US

Funding

Quasispecies dynamics in arbovirus persistence emergence and fitnessR01AI067380 · NIAID · UNIVERSITY OF NEW MEXICO · PI Gregory David Ebel · 2007 to 2026
$7.7M
Mechanism of Flavivirus RNA CappingR01AI132668 · NIAID · COLORADO STATE UNIVERSITY · PI GEISS, BRIAN · 2018 to 2022
$1.9M
NIAID NIH HHS R01 AI067380NIAID NIH HHS R01 AI132668
6 · The paper itself

Abstract

The unwinding of double-stranded RNA intermediates is critical for the replication and packaging of flavivirus RNA genomes. This unwinding activity is achieved by the ATP-dependent nonstructural protein 3 (NS3) helicase. In previous studies, we investigated the mechanism of energy transduction between the ATP and RNA binding pockets using molecular dynamics simulations and enzymatic characterization. Our data corroborated the hypothesis that motif V is a communication hub for this energy transduction. More specifically, mutations T407A and S411A in motif V exhibit a hyperactive helicase phenotype, leading to the regulation of translocation and unwinding during replication. However, the effect of these mutations on viral infection in cell culture and

Indexed as

AnimalsCell LineChlorocebus aethiopsCulexCulicidaeDEAD-box RNA HelicasesFemaleFlavivirusHEK293 CellsHumansModels, MolecularMutationNucleoside-TriphosphataseProtein Interaction Domains and MotifsRNA HelicasesSerine EndopeptidasesDEAD-box RNA HelicasesNS3 protein, flavivirusNucleoside-TriphosphataseRNA HelicasesSerine EndopeptidasesViral Nonstructural ProteinsViral Proteasescytopathic effectflavivirushelicasemosquito

Identifiers

PMID32699093
PMCPMC7495370
OpenAlexW3044180676

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.