Evidence map›Paper›PMID 32698896›Full record

ArticleGenome biology2020

Producing polished prokaryotic pangenomes with the Panaroo pipeline.

Gerry Tonkin-Hill, Neil MacAlasdair, Christopher Ruis, Aaron Weimann, Gal Horesh, John A Lees, Rebecca A Gladstone, Stephanie Lo, Christopher Beaudoin, R Andres Floto and 4 more

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Genome biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 873 papers.

0numbers the graph read from it
0cells of the map it votes in
873citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

873 citing papers in PubMed.

  1. Article
  2. Gut microbes · 2026
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  3. Dual resistance to carbapenems and colistin inEmerging microbes & infections · 2026
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  4. Article
  5. Emergence of carbapenem-resistant putative hypervirulentEmerging microbes & infections · 2026
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  6. Article
  7. Article
  8. Pangenome-GuidedPharmaceuticals (Basel, Switzerland) · 2026
    Article
  9. Article
  10. Article
  11. Whole-Genome Sequencing ofMicroorganisms · 2026
    Article
  12. ThemBio · 2026
    Article
  13. Article
  14. Article
  15. Early-life colonization withScience (New York, N.Y.) · 2026
    Article
  16. Article
  17. Article
  18. Extended-Spectrum β-Lactamase-ProducingAnimals : an open access journal from MDPI · 2026
    Article
  19. Review
  20. International journal of systematic and evolutionary microbiology · 2026
    Article

813 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Gerry Tonkin-HillParasites and Microbes, Wellcome Sanger Institute, Cambridge, UK. gt4@sanger.ac.uk.ORCID 0000-0003-4397-2224
Neil MacAlasdairParasites and Microbes, Wellcome Sanger Institute, Cambridge, UK.
Christopher RuisDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Aaron WeimannDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Gal HoreshParasites and Microbes, Wellcome Sanger Institute, Cambridge, UK.
John A LeesMRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, W2 1PG, UK.
Rebecca A GladstoneDepartment of Biostatistics, University of Oslo, Blindern, 0317, Norway.
Stephanie LoParasites and Microbes, Wellcome Sanger Institute, Cambridge, UK.
Christopher BeaudoinDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
R Andres FlotoMolecular Immunity Unit, Department of Medicine, University of Cambridge, Cambridge, UK.
Simon D W FrostMicrosoft Research, Redmond, 98052, WA, USA.
Jukka CoranderParasites and Microbes, Wellcome Sanger Institute, Cambridge, UK.
Stephen D BentleyParasites and Microbes, Wellcome Sanger Institute, Cambridge, UK.
Julian ParkhillDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.

Funding

Medical Research Council MR/R015600/1Wellcome TrustWellcome Trust 107032/Z/15/ZWellcome Trust 204016Wellcome Trust 206194
6 · The paper itself

Abstract

Population-level comparisons of prokaryotic genomes must take into account the substantial differences in gene content resulting from horizontal gene transfer, gene duplication and gene loss. However, the automated annotation of prokaryotic genomes is imperfect, and errors due to fragmented assemblies, contamination, diverse gene families and mis-assemblies accumulate over the population, leading to profound consequences when analysing the set of all genes found in a species. Here, we introduce Panaroo, a graph-based pangenome clustering tool that is able to account for many of the sources of error introduced during the annotation of prokaryotic genome assemblies. Panaroo is available at https://github.com/gtonkinhill/panaroo .

Indexed as

AlgorithmsGenome, BacterialSoftwareBiological EvolutionDrug Resistance, BacterialGenomicsKlebsiella pneumoniaeMycobacterium tuberculosisBacteriaClusteringHorizontal gene transferPangenomeProkaryote

Identifiers

PMID32698896
PMCPMC7376924

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.