Evidence map›Paper›PMID 32698761›Full record

ArticleBMC immunology2020

Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food.

Friederike Sonnet, Ellen Namork, Eva Stylianou, Ingvild Gaare-Olstad, Kanutte Huse, Sandra Andorf, Siri Mjaaland, Hubert Dirven, Unni Nygaard

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Mass Cytometry in Food Allergy Research.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Friederike SonnetDepartment of Toxicology and Risk Assessment, Norwegian Institute of Public Health, Lovisenberggata 8, Oslo, Norway. friederike-sonnet@web.de.
Ellen NamorkDepartment of Toxicology and Risk Assessment, Norwegian Institute of Public Health, Lovisenberggata 8, Oslo, Norway.
Eva StylianouRegional Unit for Asthma, Allergy and Hypersensitivity, Department of Pulmonary Diseases, Oslo University Hospital, Kirkeveien 166, Oslo, Norway.
Ingvild Gaare-OlstadRegional Unit for Asthma, Allergy and Hypersensitivity, Department of Pulmonary Diseases, Oslo University Hospital, Kirkeveien 166, Oslo, Norway.
Kanutte HuseDepartment of Cancer Immunology, Oslo University Hospital, Ullernchausseen 70, Oslo, Norway.
Sandra AndorfSean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, 291 Campus Drive, Stanford, CA, USA.
Siri MjaalandDepartment of Infectious Diseases Epidemiology and Modelling, Norwegian Institute of Public Health, Lovisenberggata 8, Oslo, Norway.
Hubert DirvenDepartment of Toxicology and Risk Assessment, Norwegian Institute of Public Health, Lovisenberggata 8, Oslo, Norway.
Unni NygaardDepartment of Toxicology and Risk Assessment, Norwegian Institute of Public Health, Lovisenberggata 8, Oslo, Norway.
Norwegian Institute of Public Health · NOOslo University Hospital · NOStanford University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe underlying cellular mechanisms causing adverse reactions to food are complex and still not fully understood. Therefore, in this study we aimed to identify functional and/or phenotypical immune cell signatures characteristic for adult patients reporting adverse reactions to food. By mass cytometry, we performed high-dimensional profiling of peripheral blood mononuclear cells (PBMC) from adult patients reporting adverse reactions to food and healthy controls. The patients were grouped according to sIgE-positive or sIgE-negative serology to common food and inhalant allergens. Two broad antibody panels were used, allowing determination of major immune cell populations in PBMC, as well as activation status, proliferation status, and cytokine expression patterns after PMA/ionomycin-stimulation on a single cell level.

resultsBy use of data-driven algorithms, several cell populations were identified showing significantly different marker expression between the groups. Most striking was an impaired frequency and function of polyfunctional CD4+ and CD8+ T cells in patients reporting adverse reactions to food compared to the controls. Further, subpopulations of monocytes, T cells, and B cells had increased expression of functional markers such as CD371, CD69, CD25, CD28, and/or HLA-DR as well as decreased expression of CD23 in the patients. Most of the differing cell subpopulations were similarly altered in the two subgroups of patients.

conclusionOur results suggest common immune cell features for both patient subgroups reporting adverse reactions to food, and provide a basis for further studies on mechanistic and diagnostic biomarker studies in food allergy.

Indexed as

AdultAllergensBiomarkersCell ProliferationCytokinesFemaleFoodFood HypersensitivityHumansImmunoglobulin ELeukocytes, MononuclearLymphocyte ActivationMaleMiddle AgedT-LymphocytesAllergensBiomarkersCytokinesImmunoglobulin EAdverse reactions to foodCITRUS algorithmCyTOF/mass cytometryFood allergyPolyfunctional cellsSpecific IgE

Identifiers

PMID32698761
PMCPMC7376650
OpenAlexW3045003275

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.