Evidence map›Paper›PMID 32698560›Full record

ArticleObstetrics & gynecology science2020

Isolation of mesenchymal stem cells from Pap smear samples.

Junghyun Park, Daryeon Son, Wonjun Hong, Jihoon Jang, Geum Joon Cho, Gwonhwa Song, In Yong Kim, Seungkwon You

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Obstetrics & gynecology science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Junghyun ParkDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Daryeon SonDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Wonjun HongDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Jihoon JangDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Geum Joon ChoDepartment of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Korea.
Gwonhwa SongDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
In Yong KimDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Seungkwon YouDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Korea University · KR

Funding

Korea Institute for Advancement of Technology N0002405,2017Korea UniversityMinistry of Health and Welfare HI15C0810Ministry of Trade, Industry and Energy
6 · The paper itself

Abstract

objectiveExploiting their ability to differentiate into mesenchymal lineages like cartilage, bone, fat, and muscle, and to elicit paracrine effects, mesenchymal stem cells (MSCs) are widely used in clinical settings to treat tissue injuries and autoimmune disorders. One of accessible sources of MSC is the samples used for Papanicolaou (Pap) test, which is a cervical screening method for detecting potentially pre-cancerous and cancerous alterations in the cervical cells and to diagnose genetic abnormalities in fetuses. This study aimed to identify and isolate the stem cells from Pap smear samples collected from pregnant women, and to trace the origin of these cells to maternal or fetal tissue, and characterize their stem cell properties.

methodsTo investigate the possibility and efficiency of establishing MSC lines from the Pap smear samples, we were able to establish 6 cell lines from Pap smear samples from 60 pregnant women at different stages of gestation.

resultsThe 3 cell lines randomly selected among the 6 established in this study, displayed high proliferation rates, several characteristics of MSCs, and the capacity to differentiate into adipocytes, osteocytes, and chondrocytes. Our study identified that the stem cell lines obtainable from Pap smear sampling were uterine cervical stromal cells (UCSCs) and had 10% efficiency of establishment.

conclusionDespite their low efficiency of establishment, human UCSCs from Pap smear samples can become a simple, safe, low-cost, and donor-specific source of MSCs for stem cell therapy and regenerative medicine.

Indexed as

Mesenchymal stem cellsPapanicolaou testRegenerative medicine

Identifiers

PMID32698560
PMCPMC7494764
OpenAlexW3044147651

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.