ArticleCell reports2020
Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family.
Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Molecular Insights on the Assembling of ParB1 and ParB1-ACS bio & med chem Au · 2026Article
- A dual-function regulatory element couples ParB expression and DNA substrate specificity.bioRxiv : the preprint server for biology · 2026Article
- Inference of fitness landscapes with heterogeneous patterns of epistasis across sites.bioRxiv : the preprint server for biology · 2026Article
- Plasma membrane invaginations and their diverse cellular functions.BMC biology · 2026Review
- Antimicrobial resistance analysis of Escherichia coli isolated from foxes in China.Veterinary research communications · 2026Article
- Inference and Visualization of Complex Genotype-Phenotype Maps.Molecular biology and evolution · 2026Article
- Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Looking at bacterial cell poles from a liquid-liquid phase separation of intrinsically disordered proteins perspective.Current research in structural biology · 2025Article
- Inference and visualization of complex genotype-phenotype maps withbioRxiv : the preprint server for biology · 2025Article
- Unveiling the multifaceted domain polymorphism of the Menshen antiphage system.Nucleic acids research · 2025Article
- How do spherical bacteria regulate cell division?Biochemical Society transactions · 2025Review
- ParSite is a multicolor DNA labeling system that allows for simultaneous imaging of triple genomic loci in living cells.PLoS biology · 2025Article
- On the Analysis of Protein Genetic Architecture: Response to "Protein sequence landscapes are not so simple".bioRxiv : the preprint server for biology · 2024Article
- The simplicity of protein sequence-function relationships.Nature communications · 2024Article
- Ancestral Reconstruction and the Evolution of Protein Energy Landscapes.Annual review of biophysics · 2024Review
- Inhibitor of Chromosome Segregation inACS chemical biology · 2024Article
- Tracking pairwise genomic loci by the ParB-ParS and Noc-NBS systems in living cells.Nucleic acids research · 2024Article
- Article
- Robust genetic codes enhance protein evolvability.PLoS biology · 2024Article
- The simplicity of protein sequence-function relationships.bioRxiv : the preprint server for biology · 2024Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Specific interactions between proteins and DNA are essential to many biological processes. Yet, it remains unclear how the diversification in DNA-binding specificity was brought about, and the mutational paths that led to changes in specificity are unknown. Using a pair of evolutionarily related DNA-binding proteins, each with a different DNA preference (ParB [Partitioning Protein B] and Noc [Nucleoid Occlusion Factor], which both play roles in bacterial chromosome maintenance), we show that specificity is encoded by a set of four residues at the protein-DNA interface. Combining X-ray crystallography and deep mutational scanning of the interface, we suggest that permissive mutations must be introduced before specificity-switching mutations to reprogram specificity and that mutational paths to new specificity do not necessarily involve dual-specificity intermediates. Overall, our results provide insight into the possible evolutionary history of ParB and Noc and, in a broader context, might be useful for understanding the evolution of other classes of DNA-binding proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.