Evidence map›Paper›PMID 32697977›Full record

ArticleBiophysical journal2020

Increased Stiffness Inhibits Invadopodia Formation and Cell Migration in 3D.

Julie Chang, Emily M Pang, Kolade Adebowale, Katrina M Wisdom, Ovijit Chaudhuri

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 48 citations in OpenAlex.

  1. Review
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  8. Hydrogel Design to Understand and Guide 3D Cell Migration.Regenerative engineering and translational medicine · 2025
    Review
  9. Mechanical signatures in cancer metastasis.npj biological physics and mechanics · 2025
    Review
  10. Article
  11. Article
  12. Review
  13. Matrix stiffness-dependent regulation of immunomodulatory genes in human MSCs is associated with the lncRNA CYTOR.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  14. Article
  15. Review
  16. Review
  17. Review
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Julie ChangDepartment of Bioengineering, Stanford University, Stanford, California.
Emily M PangDepartment of Mechanical Engineering, Stanford University, Stanford, California.
Kolade AdebowaleDepartment of Chemical Engineering, Stanford University, Stanford, California.
Katrina M WisdomDepartment of Mechanical Engineering, Stanford University, Stanford, California; Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania.
Ovijit ChaudhuriDepartment of Mechanical Engineering, Stanford University, Stanford, California. Electronic address: chaudhuri@stanford.edu.
Stanford University · USUniversity of Pennsylvania · US

Funding

Role of extracellular matrix malleability in mediating breast cancer cell invasion and migrationR37CA214136 · NCI · STANFORD UNIVERSITY · PI CHAUDHURI, OVIJIT · 2018 to 2024
$2.5M
Stanford Chem-H Chemistry/Biology Interface Predoctoral Training ProgramT32GM120007 · NIGMS · STANFORD UNIVERSITY · PI BERTOZZI, CAROLYN · 2016 to 2020
$1.2M
NCI NIH HHS R37 CA214136NIGMS NIH HHS T32 GM120007
6 · The paper itself

Abstract

Cancer cells typically invade through basement membranes (BMs) at key points during metastasis, including primary tumor invasion, intravasation, and extravasation. Cells extend invadopodia protrusions to create channels in the nanoporous BM through which they can invade, either via proteolytic degradation or mechanical force. Increased matrix stiffness can promote cancer progression, and two-dimensional (2D) culture studies indicate that increased stiffness promotes invadopodia degradation activity. However, invadopodia can function mechanically, independent of their degradative activity, and cells do not form fully matured invadopodia or migrate in the direction of the invadopodia in 2D environments. Here, we elucidated the impact of matrix stiffness on the mechanical mode of invadopodia activity of cancer cells cultured in three-dimensional BM-like matrices. Invadopodia formation and cell migration assays were performed for invasive breast cancer cells cultured in mechanically plastic, nanoporous, and minimally degradable interpenetrating networks of reconstituted BM matrix and alginate, which presented a range of elastic moduli from 0.4 to 9.3 kPa. Across this entire range of stiffness, we find that cells form mature invadopodia that often precede migration in the direction of the protrusion. However, at higher stiffness, cells form shorter and more transient invadopodia and are less likely to extend invadopodia overall, contrasting with results from 2D studies. Subsequently, cell migration is diminished in stiff environments. Thus, although previous studies indicate that increased stiffness may promote malignant phenotypes and the degradative activity of invadopodia, our findings show that increased stiffness physically restricts invadopodia extension and cell migration in three-dimensional, BM-like environments.

Indexed as

PodosomesBasement MembraneCell Line, TumorCell MovementExtracellular MatrixHumansNeoplasm Invasiveness

Identifiers

PMID32697977
PMCPMC7451915
OpenAlexW3043287223

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.