ArticleBlood advances2020
Platelet α-granules are required for occlusive high-shear-rate thrombosis.
Article in Blood advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Platelet homeostasis and heterogeneity in inflammation and sepsis: mechanisms, biological effects, and clinical potential.Cell communication and signaling : CCS · 2026Review
- Platelet dysfunction in chronic kidney disease arises from bone marrow remodeling and megakaryocyte reprogramming.Blood advances · 2026Article
- Platelet-Derived Components for Skin and Bone Aging and Age-Associated Pathologies: Mechanisms, Bioengineering Strategies, and Clinical Translation.Molecules (Basel, Switzerland) · 2026Review
- Differential Regulation of Granule Release in Platelets and Immune Cells.TH open : companion journal to thrombosis and haemostasis · 2026Article
- Arterial human blood flow can be stopped by cables of VWF and platelets without coagulation.Blood advances · 2025Article
- In vitro and patient studies with platelets to explore off-target cardiovascular effects of integrase inhibitors.HIV medicine · 2025Article
- GPVI-mediated thrombus stabilization of shear-induced platelet aggregates in a microfluidic stenosis.Biophysical journal · 2025Article
- One immune cell to bind them all: platelet contribution to neurodegenerative disease.Molecular neurodegeneration · 2024Review
- A Synergistic Overview between Microfluidics and Numerical Research for Vascular Flow and Pathological Investigations.Sensors (Basel, Switzerland) · 2024Review
- Platelet Biorheology and Mechanobiology in Thrombosis and Hemostasis: Perspectives from Multiscale Computation.International journal of molecular sciences · 2024Review
- The Crossroads of the Coagulation System and the Immune System: Interactions and Connections.International journal of molecular sciences · 2023Review
- Dose-Dependent Von Willebrand Factor Inhibition by Aptamer BB-031 Correlates with Thrombolysis in a Microfluidic Model of Arterial Occlusion.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Structure of shear-induced platelet aggregated clot formed in an in vitro arterial thrombosis model.Blood advances · 2022Article
- SIPA in 10 milliseconds: VWF tentacles agglomerate and capture platelets under high shear.Blood advances · 2022Article
- The Role of Von Willebrand Factor in the Pathogenesis of Pulmonary Vascular Thrombosis in COVID-19.Viruses · 2022Article
- Von Willebrand factor in diagnostics and treatment of cardiovascular disease: Recent advances and prospects.Frontiers in cardiovascular medicine · 2022Review
- Global Thrombosis Test: Occlusion by Coagulation or SIPA?TH open : companion journal to thrombosis and haemostasis · 2021Article
- Emerging Microfluidic Approaches for Platelet Mechanobiology and Interplay With Circulatory Systems.Frontiers in cardiovascular medicine · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
von Willebrand factor (VWF) is essential for the induction of arterial thrombosis. In this study, we investigated the critical role of platelet VWF in occlusive thrombosis formation at high shear in mice that do not express platelet VWF (Nbeal2-/-). Using in silico modeling, in vitro high-shear microfluidics, and an in vivo Folts model of arterial thrombosis we reproduced the platelet dynamics that occur under pathological flow in a stenosed vessel. Computational fluid dynamics (CFDs) simulated local hemodynamics in a stenosis based on arterial geometries. The model predicted shear rates, time course of platelet adhesion, and time to occlusion. These predictions were validated in vitro and in vivo. Occlusive thrombosis developed in wild-type control mice that had normal levels of plasma VWF and platelet VWF in vitro and in vivo. Occlusive thrombosis did not form in the Nbeal2-/- mice that had normal plasma VWF and an absence of platelet VWF. Occlusive thrombosis was corrected in Nbeal2-/- microfluidic assays by the addition of exogenous normal platelets with VWF. Combining model and experimental data, we demonstrated the necessary requirement of platelet VWF in α-granules in forming an occlusive thrombus under high shear. These results could inspire new pharmacological targets specific to pathological conditions and prevent arterial thrombosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.