ArticleBio-protocol2019
Estrous Cycle Monitoring in Mice with Rapid Data Visualization and Analysis.
Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Defining functional puberty in female C57BL/6J mice using endocrine, cytological and morphological markers.Scientific reports · 2026Article
- Microglia Rank signaling regulates GnRH neuronal function and the hypothalamic-pituitary-gonadal axis.Science (New York, N.Y.) · 2026Article
- Article
- Meteorin-like protein may be a mediator of exercise-related reproductive disorders in female rats.Scientific reports · 2026Article
- Low dose exposure to dioxins alters hepatic energy metabolism and steatotic liver disease development in a sex-specific manner.Environment international · 2024Article
- Article
- Multi-organ transcriptome atlas of a mouse model of relative energy deficiency in sport.Cell metabolism · 2024Article
- Sex-specific effects of acute chlordane exposure in the context of steatotic liver disease, energy metabolism and endocrine disruption.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2023Article
- Collection and Analysis of Vaginal Smears to Assess Reproductive Stage in Mice.Current protocols · 2023Article
- Female-specific pituitary gonadotrope dysregulation in mice with chronic focal epilepsy.Experimental neurology · 2023Article
- Sex and Estrous Cycle Stage Shape Left-Right Asymmetry in Chronic Hippocampal Seizures in Mice.eNeuro · 2023Article
- Estrous Cycle Mediates Midbrain Neuron Excitability Altering Social Behavior upon Stress.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Sex and estrous cycle stage shape left-right asymmetry in chronic hippocampal seizures in mice.bioRxiv : the preprint server for biology · 2023Article
- Evaluating the interplay between estrous cyclicity and flurothyl-induced seizure susceptibility inmicroPublication biology · 2023Article
- Automated classification of estrous stage in rodents using deep learning.Scientific reports · 2022Article
- Increased GABA transmission to GnRH neurons after intrahippocampal kainic acid injection in mice is sex-specific and associated with estrous cycle disruption.Neurobiology of disease · 2022Article
- Phenotypic differences based on lateralization of intrahippocampal kainic acid injection in female mice.Experimental neurology · 2022Article
- Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice.Nature communications · 2022Article
- Epilepsy-associated increase in gonadotropin-releasing hormone neuron firing in diestrous female mice is independent of chronic seizure burden severity.Epilepsy research · 2022Article
- Rodent Estrous Cycle Monitoring Utilizing Vaginal Lavage: No Such Thing As a Normal Cycle.Journal of visualized experiments : JoVE · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The estrous cycle provides a readout of reproductive health in female laboratory rodents, and estrous cycle stage can be an important physiological variable. Accurate assessment of estrous cycle stage is also important in producing timed pregnancies for developmental studies. Here, we provide a protocol for evaluation of estrous cycle stage through a minimally invasive procedure of acquiring cells lining the vaginal cavity and immediate microscopic visual assessment of these cells without drying or staining. When performed over several consecutive days, the pattern of progression through the four main stages of the estrous cycle, and disruptions to this pattern, can be determined. We also present software that enables more efficient cycle stage data analysis and pattern visualization. These protocols and tools will thus facilitate the incorporation of female animals in laboratory experiments and enhance the assessment of relationships between the reproductive cycle and overall physiology and behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.