ArticleScientific reports2020
Cyclic di-GMP cyclase SSFG_02181 from Streptomyces ghanaensis ATCC14672 regulates antibiotic biosynthesis and morphological differentiation in streptomycetes.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 23 citations in OpenAlex.
- Comparative Genomics and Metabolomics of Domesticated, Pladienolide-ProducingJournal of natural products · 2026Article
- Fluorescence-Based Soil Survival Analysis of the Xenobiotic- and Metal-DetoxifyingInternational journal of molecular sciences · 2025Article
- Gene sco1417 encodes a positive regulator of the de novo biosynthesis of pyridoxal 5'-phosphate (vitamin B6) in Streptomyces coelicolor M145.BMC microbiology · 2025Article
- Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces.World journal of microbiology & biotechnology · 2024Review
- Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous CyanobacteriumInternational journal of molecular sciences · 2023Article
- Streptomyces polyketides mediate bacteria-fungi interactions across soil environments.Nature microbiology · 2023Article
- Nitric Oxide Signaling for Aerial Mycelium Formation in Streptomyces coelicolor A3(2) M145.Applied and environmental microbiology · 2022Article
- Structural diversity, bioactivity, and biosynthesis of phosphoglycolipid family antibiotics: Recent advances.BBA advances · 2022Article
- Genetic Engineering ofMicroorganisms · 2021Article
- Spore-Associated Proteins Involved in c-di-GMP Synthesis and Degradation of Bacillus anthracis.Journal of bacteriology · 2021Article
- Article
- Regulatory Control of Rishirilide(s) Biosynthesis inMicroorganisms · 2021Article
- Identification and Characterization of Four c-di-GMP-Metabolizing Enzymes fromMicroorganisms · 2021Article
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4 authors at 1 institution in 1 country.
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Abstract
Streptomycetes are filamentous bacteria famous for their ability to produce a vast majority of clinically important secondary metabolites. Both complex morphogenesis and onset of antibiotic biosynthesis are tightly linked in streptomycetes and require series of specific signals for initiation. Cyclic dimeric 3'-5' guanosine monophosphate, c-di-GMP, one of the well-known bacterial second messengers, has been recently shown to govern morphogenesis and natural product synthesis in Streptomyces by altering the activity of the pleiotropic regulator BldD. Here we report a role of the heme-binding diguanylate cyclase SSFG_02181 from Streptomyces ghanaensis in the regulation of the peptidoglycan glycosyltransferase inhibitor moenomycin A biosynthesis. Deletion of ssfg_02181 reduced the moenomycin A accumulation and led to a precocious sporulation, while the overexpression of the gene blocked sporogenesis and remarkably improved antibiotic titer. We also demonstrate that BldD negatively controls the expression of ssfg_02181, which stems from direct binding of BldD to the ssfg_02181 promoter. Notably, the heterologous expression of ssfg_02181 in model Streptomyces spp. arrested morphological progression at aerial mycelium level and strongly altered the production of secondary metabolites. Altogether, our work underscores the significance of c-di-GMP-mediated signaling in natural product biosynthesis and pointed to extensively applicable approach to increase antibiotic production levels in streptomycetes.
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