ArticleG3 (Bethesda, Md.)2020
Gene Transcription as a Limiting Factor in Protein Production and Cell Growth.
Article in G3 (Bethesda, Md.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Article
- Somatic polyploidy supports biosynthesis and tissue function by increasing transcriptional output.The Journal of cell biology · 2025Article
- Noise robustness and metabolic load determine the principles of central dogma regulation.Science advances · 2024Article
- Noise robustness and metabolic load determine the principles of central dogma regulation.ArXiv · 2024Article
- Noise robustness and metabolic load determine the principles of central dogma regulation.bioRxiv : the preprint server for biology · 2024Article
- How total mRNA influences cell growth.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Transcript accumulation rates in the earlyScience advances · 2023Article
- Different activity patterns control various stages of Reelin synthesis in the developing neocortex.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- MicroRNAs as Targets for Cancer Diagnosis: Interests and Limitations.Advanced pharmaceutical bulletin · 2023Review
- Scaling of nuclear numbers and their spatial arrangement in skeletal muscle cell size regulation.Molecular biology of the cell · 2023Review
- Scaling of biosynthesis and metabolism with cell size.Molecular biology of the cell · 2022Article
- Massive expression of cysteine-containing proteins causes abnormal elongation of yeast cells by perturbing the proteasome.G3 (Bethesda, Md.) · 2022Article
- Article
- Homeostasis in the Central Dogma of molecular biology: the importance of mRNA instability.RNA biology · 2019Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell growth is driven by the synthesis of proteins, genes, and other cellular components. Defining processes that limit biosynthesis rates is fundamental for understanding the determinants of cell physiology. Here, we analyze the consequences of engineering cells to express extremely high levels of mCherry proteins, as a tool to define limiting processes that fail to adapt upon increasing biosynthetic demands. Protein-burdened cells were transcriptionally and phenotypically similar to mutants of the Mediator, a transcription coactivator complex. However, our binding data suggest that the Mediator was not depleted from endogenous promoters. Burdened cells showed an overall increase in the abundance of the majority of endogenous transcripts, except for highly expressed genes. Our results, supported by mathematical modeling, suggest that wild-type cells transcribe highly expressed genes at the maximal possible rate, as defined by the transcription machinery's physical properties. We discuss the possible cellular benefit of maximal transcription rates to allow a coordinated optimization of cell size and cell growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.